miRNA Maturation : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2014.
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| Edition: | 1st ed. 2014. |
| Series: | Methods in Molecular Biology,
1095 |
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Table of Contents:
- The Pathway of miRNA Maturation
- Micro RNA Maturation and Human Disease
- Approaches to the Modulation of miRNA Maturation
- Expression Profiling of Components of the miRNA Maturation Machinery
- Primary microRNA Processing Assay Reconstituted using Recombinant Drosha and DGCR8
- In-vivo processing Assay Based on a Dual Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity
- Assaying Dicer-mediated miRNA Maturation by Means of Fluorescent Substrates
- A Fluorescence Correlation Spectroscopy-based Enzyme Assay for Human Dicer
- Detection of Micro RNA Maturation Using Unmodified pre-miRNA and Branched Rolling Circle Amplification
- Identification of Inhibitors of microRNA Function from Small Molecule Screens
- Quantitative RT-PCR Specific for Precursor and Mature miRNAs
- Cellular MicroRNA Sensors Based on Luciferase Reporters
- Inhibition of miRNA maturation by Peptide Nucleic Acids
- Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs
- Lentiviral Overexpression of miRNAs.