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180629s2018 xxu| s |||| 0|eng d |
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|a 9781493986248
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| 024 |
7 |
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|a 10.1007/978-1-4939-8624-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a miRNA Biogenesis :
|b Methods and Protocols /
|c edited by Ulf Andersson Vang Ørom.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Springer,
|c 2018.
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| 300 |
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|a XI, 238 p. 58 illus., 45 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1823
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| 505 |
0 |
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|a High-throughput characterization of primary microRNA transcripts -- Identifying pri-miRNA transcription start sites -- Metabolic pulse-chase RNA labelling for pri-miRNA processing dynamics -- In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-way System for RNAP II Transcription/Splicing/microRNA Processing -- Purification of Microprocessor-associated factors -- Arresting pri-miRNA processing with Target Site Blockers -- MicroRNA analysis using the quantitative real-time PCR reaction -- MicroRNA analysis using next-generation sequencing -- Identification of microRNA precursor-associated proteins -- Analysis of 3' end modifications in (precursor-) microRNAs by high-throughput -- Uncovering the stability of mature miRNAs by 4-thio-uridine metabolic labeling -- Detection of microRNA-target interactions by Chimera PCR (ChimP) -- High-quality overlapping paired-end reads for the detection of A-to-I editing on small RNA -- Targeting miRNA for therapeutics using a Micronome based method for identification of miRNA-mRNA pairs and validation of key regulator miRNA -- Method for detection of miRNAs in non-model organisms with unreported database -- Detection and Verification of Mammalian Mirtrons by Northern Blotting -- Detecting agotrons in Ago CLIPseq data.
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| 650 |
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|a Human genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 700 |
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|a Ørom, Ulf Andersson Vang.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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