miRNA Biogenesis : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Springer,
2018.
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| Edición: | 1st ed. 2018. |
| Colección: | Methods in Molecular Biology,
1823 |
| Materias: |
Tabla de Contenidos:
- High-throughput characterization of primary microRNA transcripts
- Identifying pri-miRNA transcription start sites
- Metabolic pulse-chase RNA labelling for pri-miRNA processing dynamics
- In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-way System for RNAP II Transcription/Splicing/microRNA Processing
- Purification of Microprocessor-associated factors
- Arresting pri-miRNA processing with Target Site Blockers
- MicroRNA analysis using the quantitative real-time PCR reaction
- MicroRNA analysis using next-generation sequencing
- Identification of microRNA precursor-associated proteins
- Analysis of 3' end modifications in (precursor-) microRNAs by high-throughput
- Uncovering the stability of mature miRNAs by 4-thio-uridine metabolic labeling
- Detection of microRNA-target interactions by Chimera PCR (ChimP)
- High-quality overlapping paired-end reads for the detection of A-to-I editing on small RNA
- Targeting miRNA for therapeutics using a Micronome based method for identification of miRNA-mRNA pairs and validation of key regulator miRNA
- Method for detection of miRNAs in non-model organisms with unreported database
- Detection and Verification of Mammalian Mirtrons by Northern Blotting
- Detecting agotrons in Ago CLIPseq data.