Yeast Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Xiao, Wei. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2014.
Edition:3rd ed. 2014.
Series:Methods in Molecular Biology, 1163
Subjects:
Table of Contents:
  • Basic Investigations in Saccharomyces cerevisiae
  • Isolation of Yeast Nucleic Acids
  • Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry
  • Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
  • Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe
  • See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry
  • Yeast Two Hybrid Liquid Screening
  • Targeted Mutagenesis of a Specific Gene in Yeast
  • Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae
  • Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy
  • Assays for Autophagy II: Mitochondrial Autophagy
  • Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction
  • Yeast Survival and Growth Assays
  • Spontaneous Mutagenesis Assay
  • Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene
  • piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe
  • Replicative and Chronological Life Span Assays
  • Detection of Protein Arginine Methylation in Saccharomyces cerevisiae
  • Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae
  • Ligand Engineering Using Yeast Surface Display
  • Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay.