mRNA Decay : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edición: | 1st ed. 2018. |
| Colección: | Methods in Molecular Biology,
1720 |
| Materias: |
Tabla de Contenidos:
- 5'bromouridine IP Chase (BRIC)-Seq to Determine RNA Half Lives
- Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq)
- Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay
- Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH
- PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins
- Characterizing mRNA Sequence Motifs in the 3'UTR Using GFP Reporter Constructs
- iCLIP of the PIWI Protein Aubergine in Drosophila Embryos
- Integration of ENCODE RNAseq and eCLIP Data Sets
- Identifying miRNA Targets Using AgoRIP-Seq.- Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets
- Identifying RISC Components Using Ago2 Immunoprecipitation and Mass Spectrometry
- Using Tet-Off Cells and RNAi Knockdown to Assay mRNA Decay
- Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq)
- Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity
- Reactivation Assay to Identify Direct Targets of the Nonsense-Mediated mRNA Decay Pathway in Drosophila.- Studying Nonsense-Mediated mRNA Decay in Mammalian Cells Using a Multicolored Bioluminescence-Based Reporter System.