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03875nam a22003975i 4500 |
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978-1-4020-4896-8 |
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20191028092104.0 |
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cr nn 008mamaa |
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100301s2006 ne | s |||| 0|eng d |
| 020 |
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|a 9781402048968
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| 024 |
7 |
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|a 10.1007/978-1-4020-4896-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Reviews and Protocols in DT40 Research
|b Subcellular Biochemistry /
|c edited by Jean-Marie Buerstedde, Shunichi Takeda.
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| 250 |
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|a 1st ed. 2006.
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| 260 |
# |
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|a Dordrecht :
|b Springer Netherlands :
|b Imprint: Springer,
|c 2006.
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| 300 |
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|a XII, 477 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Subcellular Biochemistry,
|v 40
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| 505 |
0 |
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|a DT40 gene disruptions: a how-to for the design and the construction of targeting vectors -- Immunoglobulin gene conversion or hypermutation: that's the question -- Genome resources for the DT40 community -- Chromosome engineering in DT40 cells and mammalian centromere function -- Function of RECQ family helicase in genome stability -- Genetic analysis of apoptotic execution -- The DT40 system as a tool for analyzing kinetochore assembly -- Analysing the DNA damage and replication checkpoints in DT40 cells -- Using DT40 to study clathrin function -- Genetic analysis of B cell signaling -- DT40 mutants: a model to study transcriptional regulation of B cell development and function -- Transcription and RNA processing factors play complex roles in DT40 cells -- Participation of histones, histone modifying enzymes and histone chaperonesin vertebrate cell functions -- Analysis of gene expression, copy number and palindrome formation with a DT40 enriched CDNA microarray -- Calcium signaling, ion channels and more -- Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development -- The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line -- Phenotypic analysis of cellular responses to DNA damage -- ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells -- Stable non-targeted transfection of DT40 -- Basic cell culture conditions -- Excision of floxed-DNA sequences by transient induction of MER-CRE-MER -- Immunoglobulin gene conversion and hypermutation assay by facs -- Target screening by PCR -- Mitotic index determination by flow cytometry -- Centrifugal elutriation as a means of cell cycle phase separation and synchronisation -- Preparation of genomic DNA for microarray-based comparative genome hybridization -- Analysis of cellular Mg2+ in DT40 cells -- Transient transfection of DT40 -- Retroviral transduction of DT40 -- Colony survival assay -- Subcloning DT40 by limiting dilution -- Subnuclear immunofluorescence -- Sister chromatid exchange assay -- 2D cell cycle analysis -- Purification of tap-tagged proteins by two-step pull down from DT40 cells -- Synchronization of cells -- Targeted transfection of DT40 cells -- Luciferase reporter assay -- Indirect immunofluorescence microscopy -- Quantification of receptor-mediated endocytosis -- Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation -- Isolation of nuclear and cytoplasmic proteins from DT40 cell lines.
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| 650 |
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|a Cell biology.
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| 650 |
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0 |
|a Cancer research.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Life sciences.
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| 650 |
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|a Vertebrates.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Cancer Research.
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| 650 |
2 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Life Sciences, general.
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| 650 |
2 |
4 |
|a Vertebrates.
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| 700 |
1 |
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|a Buerstedde, Jean-Marie.
|e editor.
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| 700 |
1 |
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|a Takeda, Shunichi.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-4020-4896-8
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