Reviews and Protocols in DT40 Research Subcellular Biochemistry /
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Dordrecht :
Springer Netherlands : Imprint: Springer,
2006.
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| Edición: | 1st ed. 2006. |
| Colección: | Subcellular Biochemistry,
40 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-4020-4896-8 |
Tabla de Contenidos:
- DT40 gene disruptions: a how-to for the design and the construction of targeting vectors
- Immunoglobulin gene conversion or hypermutation: that's the question
- Genome resources for the DT40 community
- Chromosome engineering in DT40 cells and mammalian centromere function
- Function of RECQ family helicase in genome stability
- Genetic analysis of apoptotic execution
- The DT40 system as a tool for analyzing kinetochore assembly
- Analysing the DNA damage and replication checkpoints in DT40 cells
- Using DT40 to study clathrin function
- Genetic analysis of B cell signaling
- DT40 mutants: a model to study transcriptional regulation of B cell development and function
- Transcription and RNA processing factors play complex roles in DT40 cells
- Participation of histones, histone modifying enzymes and histone chaperonesin vertebrate cell functions
- Analysis of gene expression, copy number and palindrome formation with a DT40 enriched CDNA microarray
- Calcium signaling, ion channels and more
- Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development
- The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line
- Phenotypic analysis of cellular responses to DNA damage
- ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells
- Stable non-targeted transfection of DT40
- Basic cell culture conditions
- Excision of floxed-DNA sequences by transient induction of MER-CRE-MER
- Immunoglobulin gene conversion and hypermutation assay by facs
- Target screening by PCR
- Mitotic index determination by flow cytometry
- Centrifugal elutriation as a means of cell cycle phase separation and synchronisation
- Preparation of genomic DNA for microarray-based comparative genome hybridization
- Analysis of cellular Mg2+ in DT40 cells
- Transient transfection of DT40
- Retroviral transduction of DT40
- Colony survival assay
- Subcloning DT40 by limiting dilution
- Subnuclear immunofluorescence
- Sister chromatid exchange assay
- 2D cell cycle analysis
- Purification of tap-tagged proteins by two-step pull down from DT40 cells
- Synchronization of cells
- Targeted transfection of DT40 cells
- Luciferase reporter assay
- Indirect immunofluorescence microscopy
- Quantification of receptor-mediated endocytosis
- Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation
- Isolation of nuclear and cytoplasmic proteins from DT40 cell lines.