Principles and Technical Aspects of PCR Amplification
| Autores principales: | , , |
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| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Dordrecht :
Springer Netherlands : Imprint: Springer,
2008.
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| Edición: | 1st ed. 2008. |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-4020-6241-4 |
Tabla de Contenidos:
- The Polymerase Chain Reaction
- A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification
- The PCR in Practice
- The Different Types and Varieties of Nucleic Acid Target Molecules
- PCR Primers
- Deoxynucleotide Triphosphates and Buffer Components
- Taq and Other Thermostable DNA Polymerases
- Important Considerations for Typical, Quantitative and Real-Time PCR Protocols
- Analysis of PCR Amplification Products
- Ensuring PCR Quality – Laboratory Organisation, PCR Optimization and Controls
- Ensuring PCR Quality – Quality Criteria and Quality Assurance
- Variants and Adaptations of the Standard PCR Protocol
- In Situ PCR Amplification (ISA) – Major Considerations, Sample Processing and Applications.