Principles and Technical Aspects of PCR Amplification
| Main Authors: | , , |
|---|---|
| Corporate Author: | |
| Format: | eBook |
| Language: | English |
| Published: |
Dordrecht :
Springer Netherlands : Imprint: Springer,
2008.
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| Edition: | 1st ed. 2008. |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4020-6241-4 |
Table of Contents:
- The Polymerase Chain Reaction
- A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification
- The PCR in Practice
- The Different Types and Varieties of Nucleic Acid Target Molecules
- PCR Primers
- Deoxynucleotide Triphosphates and Buffer Components
- Taq and Other Thermostable DNA Polymerases
- Important Considerations for Typical, Quantitative and Real-Time PCR Protocols
- Analysis of PCR Amplification Products
- Ensuring PCR Quality - Laboratory Organisation, PCR Optimization and Controls
- Ensuring PCR Quality - Quality Criteria and Quality Assurance
- Variants and Adaptations of the Standard PCR Protocol
- In Situ PCR Amplification (ISA) - Major Considerations, Sample Processing and Applications.