Histone Deacetylases Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Sarkar, Sibaji. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1436
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-3667-0
Tabla de Contenidos:
  • A Sensitive and Flexible Assay for Determining Histone Deacetylase 1 (HDAC1) Activity
  • Detection of Sumo Modification of Endogenous Histone Deacetylase 2 (HDAC2) in Mammalian Cells
  • Analysis of Epigenetic Regulation of Hypoxia-Induced Epithelial-Mesenchymal Transition in Cancer Cells by Quantitative Chromatin Immunoprecipitation of Histone Deacetylase 3 (HDAC3)
  • Molecular and Functional Characterization of Histone Deacetylase 4 (HDAC4)
  • Approaches for Studying the Subcellular Localization, Interactions, and Regulation of Histone Deacetylase 5 (HDAC5)
  • Analysis of Expression and Functions of Histone Deacetylase 6 (HDAC 6)
  • Analysis of Histone Deacetylase 7 (HDAC7) Alternative Splicing and Its Role in Embryonic Stem Cell Differentiation toward Smooth Muscle Lineage
  • Large-Scale Overproduction and Purification of Recombinant Histone Deacetylase 8 (HDAC8) from the Human-Pathogenic Flatworm Schistosoma mansoni
  • Visualization of Histone Deacetylase 9 (HDAC9) Spatiotemporal Subcellular Localization in Primary Neuron Cultures
  • Expression and Function of Histone Deacetylase 10 (HDAC10) in B Cell Malignancies
  • Functional Analysis of Histone Deacetylase 11 (HDAC11)
  • Sirtuin1 (SIRT1) in the Acetylation of Downstream Target Proteins
  • Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2)
  • Cloning and Characterization of Sirtuin3 (SIRT3)
  • Identification of Sirtuin4 (SIRT4) Protein Interactions: Uncovering Candidate Acyl-Modified Mitochondrial Substrates and Enzymatic Regulators
  • Generation and Purification of Catalytically Active Recombinant Sirtuin5 (SIRT5) Protein
  • Sirtuin6 (SIRT6) Activity Assays
  • Molecular, Cellular, and Physiological Characterization of Sirtuin7 (SIRT7)
  • HDAC Inhibitors
  • Assessment of the Anti-Proliferative Activity of a BET Bromodomain Inhibitor as Single Agent and in Combination in Non-Hodgkin Lymphoma Cell Lines
  • Screening the Impact of Sirtuin Inhibitors on Inflammatory and Innate Immune Responses of Macrophages and in a Mouse Model of Endotoxic Shock. .