|
|
|
|
| LEADER |
03375nam a22003015i 4500 |
| 001 |
978-1-4939-6700-1 |
| 005 |
20191023152327.0 |
| 007 |
cr nn 008mamaa |
| 008 |
161214s2017 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781493967001
|
| 024 |
7 |
|
|a 10.1007/978-1-4939-6700-1
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Hepatitis B Virus
|b Methods and Protocols /
|c edited by Haitao Guo, Andrea Cuconati.
|
| 250 |
|
|
|a 1st ed. 2017.
|
| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
|
| 300 |
|
|
|a XII, 297 p. 57 illus., 31 illus. in color.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 1540
|
| 505 |
0 |
|
|a NTCP-Reconstituted In Vitro HBV Infection System -- Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells and Primary Human Hepatocytes -- Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells -- Intracytoplasmic Transport of Hepatitis B Virus Capsids -- A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein -- Analyses of HBV cccDNA Quantification and Modification -- Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation Specific PCR -- A T7 Endonuclease I Assay to Detect TALEN-Mediated Mutation of HBV cccDNA -- Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA using Inverse Nested PCR -- Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization -- Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice -- Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes -- In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase -- In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors -- Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay -- Microtiter-Format Assays for HBV Antigen Quantitation in Non-Clinical Applications -- Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform -- Generation of Replication Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization -- Hydrodynamic HBV Transfection Mouse Model -- An ELISPOT-Based Assay to Measure HBV-Specific CD8+ T Cell Responses in Immunocompetent Mice -- Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells and Kupffer Cells -- Partial Hepatectomy and Castration of HBV Transgenic Mice -- Studying HBV Infection and Therapy in Immune Deficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (Fah) Knockout Mice Transplanted with Human Hepatocytes -- Measurement of Antiviral Effect and Innate Immune Response during Treatment of Primary Woodchuck Hepatocytes.
|
| 650 |
|
0 |
|a Infectious diseases.
|
| 650 |
1 |
4 |
|a Infectious Diseases.
|
| 700 |
1 |
|
|a Guo, Haitao.
|e editor.
|
| 700 |
1 |
|
|a Cuconati, Andrea.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-4939-6700-1
|