Hepatitis B Virus Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Guo, Haitao. (Editor ), Cuconati, Andrea. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1540
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-6700-1
Tabla de Contenidos:
  • NTCP-Reconstituted In Vitro HBV Infection System
  • Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells and Primary Human Hepatocytes
  • Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells
  • Intracytoplasmic Transport of Hepatitis B Virus Capsids
  • A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein
  • Analyses of HBV cccDNA Quantification and Modification
  • Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation Specific PCR
  • A T7 Endonuclease I Assay to Detect TALEN-Mediated Mutation of HBV cccDNA
  • Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA using Inverse Nested PCR
  • Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization
  • Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice
  • Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes
  • In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase
  • In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors
  • Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay
  • Microtiter-Format Assays for HBV Antigen Quantitation in Non-Clinical Applications
  • Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform
  • Generation of Replication Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
  • Hydrodynamic HBV Transfection Mouse Model
  • An ELISPOT-Based Assay to Measure HBV-Specific CD8+ T Cell Responses in Immunocompetent Mice
  • Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells and Kupffer Cells
  • Partial Hepatectomy and Castration of HBV Transgenic Mice
  • Studying HBV Infection and Therapy in Immune Deficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (Fah) Knockout Mice Transplanted with Human Hepatocytes
  • Measurement of Antiviral Effect and Innate Immune Response during Treatment of Primary Woodchuck Hepatocytes.