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03435nam a22002895i 4500 |
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978-1-4939-6887-9 |
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20190617151452.0 |
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cr nn 008mamaa |
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170503s2017 xxu| s |||| 0|eng d |
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|a 9781493968879
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|a 10.1007/978-1-4939-6887-9
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Heterologous Gene Expression in E.coli
|b Methods and Protocols /
|c edited by Nicola A. Burgess-Brown.
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|a 1st ed. 2017.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XIV, 429 p. 92 illus., 55 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1586
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| 505 |
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|a Recombinant Protein Expression in E. coli: A Historical Perspective -- N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools -- Harnessing the Profinity eXactTM System for Expression and Purification of Heterologous Proteins in E. coli -- ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences -- Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters -- Optimization of Membrane Protein Production Using Titratable Strains of E. coli -- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions -- High Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B -- A Generic Protocol for Purifying Disulphide-Bonded Domains and Random Protein Fragments Using Fusion Proteins with SUMO3 and Cleavage by SenP2 Protease -- A Strategy for Production of Correctly Folded Disulfide-Rich Peptides in the Periplasm of E. coli -- Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. coli: A Tool to Engineer Stability in a LAT Transporter -- Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality -- Protein Folding Using a Vortex Fluidic Device -- Removal of Affinity Tags with TEV Protease -- Generation of Recombinant N-Linked Glycoproteins in E. coli -- Production of Protein Kinases in E. coli -- Expression of Prokaryotic Integral Membrane Proteins in E. coli -- Multiprotein Complex Production in E. coli: The SecYEG-SecDFYajC-YidC Holotranslocon -- Membrane Protein Production in E. coli Lysates in Presence of Pre-Assembled Nanodiscs -- Not Limited to E. coli: Versatile Expression Vectors for Mammalian Protein Expression -- A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis -- In Vivo Biotinylation of Antigens in E. coli -- Cold-Shock Expression System in E. coli for Protein NMR Studies -- High-Throughput Production of Proteins in E. coli for Structural Studies -- Mass Spectrometric Analysis of Proteins -- How to Determine Interdependencies of Glucose and Lactose Uptake Rates for Heterologous Protein Production with E. coli -- Interfacing Biocompatible Reactions with Engineered Escherichia coli.
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| 650 |
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|a Microbiology.
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| 650 |
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4 |
|a Microbiology.
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| 700 |
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|a Burgess-Brown, Nicola A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-4939-6887-9
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