Heterologous Gene Expression in E.coli Methods and Protocols /
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edition: | 1st ed. 2017. |
| Series: | Methods in Molecular Biology,
1586 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4939-6887-9 |
Table of Contents:
- Recombinant Protein Expression in E. coli: A Historical Perspective
- N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools
- Harnessing the Profinity eXactTM System for Expression and Purification of Heterologous Proteins in E. coli
- ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences
- Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters
- Optimization of Membrane Protein Production Using Titratable Strains of E. coli
- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions
- High Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B
- A Generic Protocol for Purifying Disulphide-Bonded Domains and Random Protein Fragments Using Fusion Proteins with SUMO3 and Cleavage by SenP2 Protease
- A Strategy for Production of Correctly Folded Disulfide-Rich Peptides in the Periplasm of E. coli
- Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. coli: A Tool to Engineer Stability in a LAT Transporter
- Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality
- Protein Folding Using a Vortex Fluidic Device
- Removal of Affinity Tags with TEV Protease
- Generation of Recombinant N-Linked Glycoproteins in E. coli
- Production of Protein Kinases in E. coli
- Expression of Prokaryotic Integral Membrane Proteins in E. coli
- Multiprotein Complex Production in E. coli: The SecYEG-SecDFYajC-YidC Holotranslocon
- Membrane Protein Production in E. coli Lysates in Presence of Pre-Assembled Nanodiscs
- Not Limited to E. coli: Versatile Expression Vectors for Mammalian Protein Expression
- A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis
- In Vivo Biotinylation of Antigens in E. coli
- Cold-Shock Expression System in E. coli for Protein NMR Studies
- High-Throughput Production of Proteins in E. coli for Structural Studies
- Mass Spectrometric Analysis of Proteins
- How to Determine Interdependencies of Glucose and Lactose Uptake Rates for Heterologous Protein Production with E. coli
- Interfacing Biocompatible Reactions with Engineered Escherichia coli.