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02740nam a22003015i 4500 |
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978-1-4939-7165-7 |
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20191029021154.0 |
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cr nn 008mamaa |
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170612s2017 xxu| s |||| 0|eng d |
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|a 9781493971657
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|a 10.1007/978-1-4939-7165-7
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Plant Argonaute Proteins
|b Methods and Protocols /
|c edited by Alberto Carbonell.
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|a 1st ed. 2017.
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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|a XI, 297 p. 47 illus., 27 illus. in color.
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1640
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|a Plant ARGONAUTEs: Features, Functions and Unknowns -- Analysis of the Uridylation of Both ARGONAUTE–Bound MiRNAs and 5’ Cleavage Products of their Target RNAs in Plants -- In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts -- In vitro Analysis of ARGONAUTE–Mediated Target Cleavage and Translational Repression in Plants -- Establishment of an In Vivo ARGONAUTE Reporter System in Plants -- Immunoprecipitation and High–throughput Sequencing of ARGONAUTE–Bound RNAs from Plants -- Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing -- Dissecting the Sub–Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA–Directed DNA Methylation Pathway in Plants -- Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana -- Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair -- Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas -- ARGONAUTE genes in Salvia miltiorrhiza: Identification, Characterization and Genetic Transformation -- Cloning and Characterization of ARGONAUTE Genes in Tomato -- Non–Radioactive Detection of Small RNAs Using Digoxygenin–Labeled Probes -- Non–Radioactive Plant Small RNA Detection Using Biotin–Labeled Probes -- Computational Analysis of Genome–Wide ARGONAUTE–Dependent DNA -- Structural and Functional Characterization of Plant ARGONAUTE MID Domains -- Identification and Analysis of WG/GW ARGONAUTE–Binding Domains -- Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms -- Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES.
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|a Plant science.
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|a Botany.
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|a Plant Sciences.
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|a Carbonell, Alberto.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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|u https://doi.org/10.1007/978-1-4939-7165-7
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