Plant Argonaute Proteins Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Carbonell, Alberto. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1640
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-7165-7
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245 1 0 |a Plant Argonaute Proteins  |b Methods and Protocols /  |c edited by Alberto Carbonell. 
250 |a 1st ed. 2017. 
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300 |a XI, 297 p. 47 illus., 27 illus. in color.  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 1640 
505 0 |a Plant ARGONAUTEs: Features, Functions and Unknowns -- Analysis of the Uridylation of Both ARGONAUTE–Bound MiRNAs and 5’ Cleavage Products of their Target RNAs in Plants -- In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts -- In vitro Analysis of ARGONAUTE–Mediated Target Cleavage and Translational Repression in Plants -- Establishment of an In Vivo ARGONAUTE Reporter System in Plants -- Immunoprecipitation and High–throughput Sequencing of ARGONAUTE–Bound RNAs from Plants -- Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing -- Dissecting the Sub–Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA–Directed DNA Methylation Pathway in Plants -- Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana -- Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair -- Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas -- ARGONAUTE genes in Salvia miltiorrhiza: Identification, Characterization and Genetic Transformation -- Cloning and Characterization of ARGONAUTE Genes in Tomato -- Non–Radioactive Detection of Small RNAs Using Digoxygenin–Labeled Probes -- Non–Radioactive Plant Small RNA Detection Using Biotin–Labeled Probes -- Computational Analysis of Genome–Wide ARGONAUTE–Dependent DNA -- Structural and Functional Characterization of Plant ARGONAUTE MID Domains -- Identification and Analysis of WG/GW ARGONAUTE–Binding Domains -- Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms -- Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES. 
650 0 |a Plant science. 
650 0 |a Botany. 
650 1 4 |a Plant Sciences. 
700 1 |a Carbonell, Alberto.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-1-4939-7165-7