Plant Argonaute Proteins Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Carbonell, Alberto. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1640
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-7165-7
Tabla de Contenidos:
  • Plant ARGONAUTEs: Features, Functions and Unknowns
  • Analysis of the Uridylation of Both ARGONAUTE–Bound MiRNAs and 5’ Cleavage Products of their Target RNAs in Plants
  • In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
  • In vitro Analysis of ARGONAUTE–Mediated Target Cleavage and Translational Repression in Plants
  • Establishment of an In Vivo ARGONAUTE Reporter System in Plants
  • Immunoprecipitation and High–throughput Sequencing of ARGONAUTE–Bound RNAs from Plants
  • Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
  • Dissecting the Sub–Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA–Directed DNA Methylation Pathway in Plants
  • Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana
  • Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
  • Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas
  • ARGONAUTE genes in Salvia miltiorrhiza: Identification, Characterization and Genetic Transformation
  • Cloning and Characterization of ARGONAUTE Genes in Tomato
  • Non–Radioactive Detection of Small RNAs Using Digoxygenin–Labeled Probes
  • Non–Radioactive Plant Small RNA Detection Using Biotin–Labeled Probes
  • Computational Analysis of Genome–Wide ARGONAUTE–Dependent DNA
  • Structural and Functional Characterization of Plant ARGONAUTE MID Domains
  • Identification and Analysis of WG/GW ARGONAUTE–Binding Domains
  • Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
  • Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES.