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03033nam a22002895i 4500 |
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978-1-59259-046-9 |
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20190617201233.0 |
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cr nn 008mamaa |
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100301s2001 xxu| s |||| 0|eng d |
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|a 9781592590469
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7 |
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|a 10.1385/1592590462
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Matrix Metalloproteinase Protocols
|c edited by Ian M. Clark.
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| 250 |
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|a 1st ed. 2001.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
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| 300 |
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|a XV, 545 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 151
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|a MMPs and TIMPs—An Overview of the Field -- MMPs and TIMPs -- Strategies for Cloning New MMPs and TIMPs -- Structural Studies on MMPs andTIMPs Wolfram Bode and Klaus Maskos -- Matrix MetaIIoproteinase Substrate Binding Domains, Modules and Exosites -- The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes -- Models for Gain-of-Function and Loss-of-Function of MMPs -- Expression and Purification of MMPs and TIMPs -- Expression of MMPs andTIMPs in Mammalian Cells -- Expression of Recombinant Matrix Metalloproteinases in Escherichia coli -- Expression of Human Collagenase I (MMP-1) andTIMP-1 in a Baculovirus-Based Expression System -- Expression of Recombinant Matrix Metalloproteinases in Yeast -- Expression of Recombinant Membrane-Type MMPs -- Refolding of TIMP-2 from Escherichia coli Inclusion Bodies -- Expression and Refolding of Full-Length HumanTIMP-1 -- Purification of MMPs and TIMPs -- Detection of MMPs and TIMPs -- Monitoring MMP andTIMP mRNA Expression by RT-PCR -- Measuring Transcription of Metalloproteinase Genes -- In Situ Hybridization for Metalloproteinases and Their Inhibitors -- Use of EIA to Measure MMPs and TIMPs -- Immunohistochemistry of MMPs and TIMPs -- Detecting Polymorphisms in MMP Genes -- Assay of MMP and TIMP Activities -- Methods for Studying Activation of Matrix Metalloproteinases -- Assay of Matrix Metalloproteinases Against Matrix Substrates -- Zymography and Reverse Zymography for Detecting MMPs, andTIMPs -- In Situ Zymography -- Detection of Focal Proteolysis Using Texas-Red-Gelatin -- Antibodies to MMP-Cleaved Aggrecan -- Cartilage Proteoglycan Release Assay -- Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens -- Collagen Degradation Assays -- Invasion Assays and Matrix Metalloproteinases -- Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases -- Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloproteinases (TIMP) -- Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Clark, Ian M.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592590462
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