Matrix Metalloproteinase Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Edición: | 1st ed. 2001. |
| Colección: | Methods in Molecular Biology,
151 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592590462 |
Tabla de Contenidos:
- MMPs and TIMPs—An Overview of the Field
- MMPs and TIMPs
- Strategies for Cloning New MMPs and TIMPs
- Structural Studies on MMPs andTIMPs Wolfram Bode and Klaus Maskos
- Matrix MetaIIoproteinase Substrate Binding Domains, Modules and Exosites
- The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes
- Models for Gain-of-Function and Loss-of-Function of MMPs
- Expression and Purification of MMPs and TIMPs
- Expression of MMPs andTIMPs in Mammalian Cells
- Expression of Recombinant Matrix Metalloproteinases in Escherichia coli
- Expression of Human Collagenase I (MMP-1) andTIMP-1 in a Baculovirus-Based Expression System
- Expression of Recombinant Matrix Metalloproteinases in Yeast
- Expression of Recombinant Membrane-Type MMPs
- Refolding of TIMP-2 from Escherichia coli Inclusion Bodies
- Expression and Refolding of Full-Length HumanTIMP-1
- Purification of MMPs and TIMPs
- Detection of MMPs and TIMPs
- Monitoring MMP andTIMP mRNA Expression by RT-PCR
- Measuring Transcription of Metalloproteinase Genes
- In Situ Hybridization for Metalloproteinases and Their Inhibitors
- Use of EIA to Measure MMPs and TIMPs
- Immunohistochemistry of MMPs and TIMPs
- Detecting Polymorphisms in MMP Genes
- Assay of MMP and TIMP Activities
- Methods for Studying Activation of Matrix Metalloproteinases
- Assay of Matrix Metalloproteinases Against Matrix Substrates
- Zymography and Reverse Zymography for Detecting MMPs, andTIMPs
- In Situ Zymography
- Detection of Focal Proteolysis Using Texas-Red-Gelatin
- Antibodies to MMP-Cleaved Aggrecan
- Cartilage Proteoglycan Release Assay
- Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens
- Collagen Degradation Assays
- Invasion Assays and Matrix Metalloproteinases
- Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases
- Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloproteinases (TIMP)
- Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases.