|
|
|
|
| LEADER |
02958nam a22002895i 4500 |
| 001 |
978-1-59259-059-9 |
| 005 |
20190617192724.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100715s2001 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781592590599
|
| 024 |
7 |
|
|a 10.1385/1592590594
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Protein Kinase Protocols
|c edited by Alastair D. Reith.
|
| 250 |
|
|
|a 1st ed. 2001.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
|
| 300 |
|
|
|a XIII, 365 p.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 124
|
| 505 |
0 |
|
|a Protein Kinase-Mediated Signaling Networks -- Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies -- Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins -- Two-Dimensional Phosphoamino Acid Analysis -- Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases -- Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry -- Phosphorylation of Smad Signaling Proteins by Receptor Serine/Threonine Kinases -- Assays for Mitogen-Activated Protein Kinase (MARK) Subtypes and MARK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate -- JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins -- Assays for Glycogen Synthase Kinase-3(GSK-3) -- Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors -- Protein Histidine Kinase -- Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System -- Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins -- Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay -- Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries -- Identification of Receptor Tyrosine Kinase (RTK) Substrates by the Cloning of ReceptorTargets (CORT) Strategy -- Identification of Receptor Tyrosine Kinase Associating Proteins Using the Yeast Two-Hybrid System -- cDNA Expression Cloning and Characterization of Phosphorylation Dependent Protein Interactors Using the Yeast Tribrid System -- Analysis of SH2 Domain - Phosphopeptide Interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance -- Cloning and Characterization of RTK Ligands Using Receptor-Alkaline Phosphatase Fusion Proteins -- Isolation and Characterization of “Orphan-RTK” Ligands Using an Integrated Biosensor Approach.
|
| 650 |
|
0 |
|a Biochemistry.
|
| 650 |
1 |
4 |
|a Biochemistry, general.
|
| 700 |
1 |
|
|a Reith, Alastair D.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/1592590594
|