Protein Kinase Protocols
| Autor Corporativo: | |
|---|---|
| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
|
| Edición: | 1st ed. 2001. |
| Colección: | Methods in Molecular Biology,
124 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592590594 |
Tabla de Contenidos:
- Protein Kinase-Mediated Signaling Networks
- Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies
- Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins
- Two-Dimensional Phosphoamino Acid Analysis
- Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases
- Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry
- Phosphorylation of Smad Signaling Proteins by Receptor Serine/Threonine Kinases
- Assays for Mitogen-Activated Protein Kinase (MARK) Subtypes and MARK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate
- JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins
- Assays for Glycogen Synthase Kinase-3(GSK-3)
- Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors
- Protein Histidine Kinase
- Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System
- Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins
- Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay
- Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries
- Identification of Receptor Tyrosine Kinase (RTK) Substrates by the Cloning of ReceptorTargets (CORT) Strategy
- Identification of Receptor Tyrosine Kinase Associating Proteins Using the Yeast Two-Hybrid System
- cDNA Expression Cloning and Characterization of Phosphorylation Dependent Protein Interactors Using the Yeast Tribrid System
- Analysis of SH2 Domain - Phosphopeptide Interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance
- Cloning and Characterization of RTK Ligands Using Receptor-Alkaline Phosphatase Fusion Proteins
- Isolation and Characterization of “Orphan-RTK” Ligands Using an Integrated Biosensor Approach.