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02430nam a22002895i 4500 |
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978-1-59259-060-5 |
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20190617192733.0 |
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cr nn 008mamaa |
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100301s2001 xxu| s |||| 0|eng d |
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|a 9781592590605
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|a 10.1385/1592590608
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Neurotrophin Protocols
|c edited by Robert A. Rush.
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| 250 |
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|a 1st ed. 2001.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
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| 300 |
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|a X, 274 p. 41 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 169
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|a Neurotrophin Quantification and Localization -- Localization of Neurotrophin Proteins Within the Central Nervous System by Immunohistochemistry -- Neurotrophin Immunohistochemistry in Peripheral Tissues -- Extraction and Quantification of the Neurotrophins -- Identifying Novel Proteins in Nervous Tissue Using Microsequencing Techniques -- RNA Quantification and Localization -- Quantification of mRNA Levels Using Northern Blotting -- Quantification of mRNA Levels Using Ribonuclease Protection Assay -- Quantification of Neurotrophin mRNA by RT-PCR -- Sensitive and Nonradioactive In Situ Detection of Neurotrophin mRNAs in the Nervous System -- Construction of Recombinant Proteins,and Transgenic Cells and Animals -- Expression of Neurotrophic Factors such as GDNF in the E. coli System -- Construction of Cells Expressing Neurotrophins -- Efficient Generation of Stable Pheochromocytoma (PC12) Cell Lines Using a Recombinant Retrovirus (LNC) -- Construction and Analysis of Transgenic Animals Overexpressing Neurotrophins -- Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors -- In Vivo Analysis -- Tracing with Radiolabeled Neurotrophins -- Toxin-Induced Death of Neurotrophin-Sensitive Neurons -- Oligonucleotides as Inhibitors of Protein Synthesis -- The IODO-GEN Method for Labeling and the Use of Retrograde Axonal Transport to Assay Neurotrophins -- Stereological Estimation of Neuronal Number The Optical Disector/Cavalieri Combination.
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| 650 |
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|a Neurosciences.
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|a Neurosciences.
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| 700 |
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|a Rush, Robert A.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592590608
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