Neurotrophin Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Edición: | 1st ed. 2001. |
| Colección: | Methods in Molecular Biology,
169 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592590608 |
Tabla de Contenidos:
- Neurotrophin Quantification and Localization
- Localization of Neurotrophin Proteins Within the Central Nervous System by Immunohistochemistry
- Neurotrophin Immunohistochemistry in Peripheral Tissues
- Extraction and Quantification of the Neurotrophins
- Identifying Novel Proteins in Nervous Tissue Using Microsequencing Techniques
- RNA Quantification and Localization
- Quantification of mRNA Levels Using Northern Blotting
- Quantification of mRNA Levels Using Ribonuclease Protection Assay
- Quantification of Neurotrophin mRNA by RT-PCR
- Sensitive and Nonradioactive In Situ Detection of Neurotrophin mRNAs in the Nervous System
- Construction of Recombinant Proteins,and Transgenic Cells and Animals
- Expression of Neurotrophic Factors such as GDNF in the E. coli System
- Construction of Cells Expressing Neurotrophins
- Efficient Generation of Stable Pheochromocytoma (PC12) Cell Lines Using a Recombinant Retrovirus (LNC)
- Construction and Analysis of Transgenic Animals Overexpressing Neurotrophins
- Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors
- In Vivo Analysis
- Tracing with Radiolabeled Neurotrophins
- Toxin-Induced Death of Neurotrophin-Sensitive Neurons
- Oligonucleotides as Inhibitors of Protein Synthesis
- The IODO-GEN Method for Labeling and the Use of Retrograde Axonal Transport to Assay Neurotrophins
- Stereological Estimation of Neuronal Number The Optical Disector/Cavalieri Combination.