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02341nam a22002895i 4500 |
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978-1-59259-069-8 |
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20191026171349.0 |
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cr nn 008mamaa |
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100301s2001 xxu| s |||| 0|eng d |
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|a 9781592590698
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| 024 |
7 |
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|a 10.1385/1592590691
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Kinesin Protocols
|c edited by Isabelle Vernos.
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| 250 |
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|a 1st ed. 2001.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
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| 300 |
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|a XI, 258 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 164
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| 505 |
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|a Purification of Kinesin from the Brain -- RT-PCR for the Identification of Developmentally Regulated Novel Members of the Kinesin-like Superfamily -- Expression Cloning with Pan Kinesin Antibodies -- Expression of Kinesin in Escherichia coli -- Plasmids for Expression of Chimeric and Truncated Kinesin Proteins -- Preparation of Recombinant Kinesin Superfamily Proteins Using the Baculovirus System -- Assays for Kinesin Microtubule-Stimulated AT Pase Activity -- An Improved Microscope for Bead and Surface-Based Motility Assays -- Use of Photonic Force Microscopy to Study Single-Motor-Molecule Mechanics -- Assays for Microtubule-Destabilizing Kinesins -- Green Fluorescent Protein as a Tag for Molecular Motor Proteins -- In Vitro Reconstitution of Endosome Motility Along Microtubules -- Approaches to Study Interactions Between Kinesin Motors and Membranes -- Microinjection Methods for Analyzing the Functions of Kinesins in Early Embryos -- The Use of Dominant Negative Mutants to Study the Function of Mitotic Motors in the In Vitro Spindle Assembly Assay in Xenopus Egg Extracts -- A Dominant Negative Approach for Functional Studies of the Kinesin II Complex -- Identification of Kinesin-Associated Proteins -- Assaying Spatial Organization of Microtubules by Kinesin Motors -- Crystallization of Kinesin -- Structural Analysis of the Microtubule–Kinesin Complex by Cryo-Electron Microscopy.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Vernos, Isabelle.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
4 |
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|u https://doi.org/10.1385/1592590691
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