Kinesin Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Edición: | 1st ed. 2001. |
| Colección: | Methods in Molecular Biology,
164 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592590691 |
Tabla de Contenidos:
- Purification of Kinesin from the Brain
- RT-PCR for the Identification of Developmentally Regulated Novel Members of the Kinesin-like Superfamily
- Expression Cloning with Pan Kinesin Antibodies
- Expression of Kinesin in Escherichia coli
- Plasmids for Expression of Chimeric and Truncated Kinesin Proteins
- Preparation of Recombinant Kinesin Superfamily Proteins Using the Baculovirus System
- Assays for Kinesin Microtubule-Stimulated AT Pase Activity
- An Improved Microscope for Bead and Surface-Based Motility Assays
- Use of Photonic Force Microscopy to Study Single-Motor-Molecule Mechanics
- Assays for Microtubule-Destabilizing Kinesins
- Green Fluorescent Protein as a Tag for Molecular Motor Proteins
- In Vitro Reconstitution of Endosome Motility Along Microtubules
- Approaches to Study Interactions Between Kinesin Motors and Membranes
- Microinjection Methods for Analyzing the Functions of Kinesins in Early Embryos
- The Use of Dominant Negative Mutants to Study the Function of Mitotic Motors in the In Vitro Spindle Assembly Assay in Xenopus Egg Extracts
- A Dominant Negative Approach for Functional Studies of the Kinesin II Complex
- Identification of Kinesin-Associated Proteins
- Assaying Spatial Organization of Microtubules by Kinesin Motors
- Crystallization of Kinesin
- Structural Analysis of the Microtubule–Kinesin Complex by Cryo-Electron Microscopy.