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02532nam a22003015i 4500 |
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978-1-59259-147-3 |
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20191026171438.0 |
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cr nn 008mamaa |
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100301s2001 xxu| s |||| 0|eng d |
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|a 9781592591473
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024 |
7 |
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|a 10.1385/1592591477
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Mycobacterium Tuberculosis Protocols
|c edited by Tanya Parish, Neil G. Stoker.
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250 |
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|a 1st ed. 2001.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
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|a XIV, 406 p.
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Medicine,
|v 54
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|a Plasmid Vectors -- Isolation of DNA from Mycobacterium tubercolosis -- Extraction of RNA from Intracellular Mycobacterium tuberculosis -- Transposon Mutagenesis in Mycobacteria Using Conditionally Replicating Mycobacteriophages -- Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB -- Gene Replacement using Pretreated DNA -- Gene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL as a Dominant Negative Selectable Marker -- Transcription Start-Site Mapping -- Fluorescence and Brightfield Cytology of Live M. tuberculosis Cells -- Phage Replication Technology for Diagnosis and Drug Susceptibility Testing -- Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy -- Restriction Fragment Length Polymorphism Typing of Mycobacteria -- Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis -- Analysis of the Capsule of Mycobacterium tuberculosis -- Analysis of the Lipids of Mycobacterium tuberculosis -- In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosis -- 17 Macrophage Virulence Assays -- Analysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell Fractionation -- Real Time PCR Using Molecular Beacons -- Electronic Access to Mycobacterium tuberculosis Sequence Data -- Proteomics -- Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays -- Storage of Mycobacterial Strains -- Safety in the Laboratory.
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650 |
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|a Infectious diseases.
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650 |
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|a Infectious Diseases.
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|a Parish, Tanya.
|e editor.
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700 |
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|a Stoker, Neil G.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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856 |
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|u https://doi.org/10.1385/1592591477
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