Mycobacterium Tuberculosis Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Parish, Tanya. (Editor ), Stoker, Neil G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2001.
Edición:1st ed. 2001.
Colección:Methods in Molecular Medicine, 54
Materias:
Acceso en línea:https://doi.org/10.1385/1592591477
Tabla de Contenidos:
  • Plasmid Vectors
  • Isolation of DNA from Mycobacterium tubercolosis
  • Extraction of RNA from Intracellular Mycobacterium tuberculosis
  • Transposon Mutagenesis in Mycobacteria Using Conditionally Replicating Mycobacteriophages
  • Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB
  • Gene Replacement using Pretreated DNA
  • Gene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL as a Dominant Negative Selectable Marker
  • Transcription Start-Site Mapping
  • Fluorescence and Brightfield Cytology of Live M. tuberculosis Cells
  • Phage Replication Technology for Diagnosis and Drug Susceptibility Testing
  • Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy
  • Restriction Fragment Length Polymorphism Typing of Mycobacteria
  • Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis
  • Analysis of the Capsule of Mycobacterium tuberculosis
  • Analysis of the Lipids of Mycobacterium tuberculosis
  • In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosis
  • 17 Macrophage Virulence Assays
  • Analysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell Fractionation
  • Real Time PCR Using Molecular Beacons
  • Electronic Access to Mycobacterium tuberculosis Sequence Data
  • Proteomics
  • Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays
  • Storage of Mycobacterial Strains
  • Safety in the Laboratory.