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04591nam a22002895i 4500 |
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978-1-59259-209-8 |
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20190617193917.0 |
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cr nn 008mamaa |
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100301s2001 xxu| s |||| 0|eng d |
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|a 9781592592098
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| 024 |
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|a 10.1385/1592592090
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Proteoglycan Protocols
|c edited by Renato V. Iozzo.
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| 250 |
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|a 1st ed. 2001.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
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| 300 |
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|a XVI, 558 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 171
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|a Isolation and Purification -- Isolation of Proteoglycans from Cell Cultures and Tissues -- Isolation of Proteoglycans from Tendon -- Purification of Proteoglycans from Mineralized Tissues -- Purification of Perlecan from Endothelial Cells -- Isolation and Characterization of Nervous Tissue Proteoglycans -- Analysis of Proteoglycans and Glycosaminoglycans from Drosophila -- Cartilage and Smooth Muscle Cell Proteoglycans Detected by Affinity Blotting Using Biotinylated Hyaluronan -- Preparation of Proteoglycans for N-Terminal and Internal Amino Acid Sequence Analysis -- High-Specific-Activity 35S-Labeled Heparan Sulfate Prepared from Cultured Cells -- Selective Detection of Sulfotransferase Isoforms by the Ligand Affinity-Conversion Approach -- Particulate and Soluble Glycosaminoglycan-Synthesizing Enzymes -- Disaccharide Composition of Hyaluronan and Chondroitin/Dermatan Sulfate -- Integral Glycan Sequencing of Heparan Sulfate and Heparin Saccharides -- Analytical and Preparative Strong Anion-Exchange HPLC of Heparan Sulfate and Heparin Saccharides -- Proteoglycans Analyzed by Composite Gel Electrophoresis and Immunoblotting -- Quantitation of Proteoglycans in Biological Fluids Using Alcian Blue -- Cellulose Acetate Electrophoresis of Glycosaminoglycans -- Disaccharide Composition in Glycosaminoglycans/Proteoglycans Analyzed by Capillary Zone Electrophoresis -- Intact and Oligomeric Glycosaminoglycans Analyzed with Capillary Electrophoresis -- Expression, Detection, and Degradation -- Recombinant Expression of Proteoglycans in Mammalian Cells -- Purification of Recombinant Human Decorin and Its Subdomains -- Prokaryotic Expression of Proteoglycans -- Proteoglycan Gene Targeting in Somatic Cells -- Constitutive and Inducible Antisense Expression -- Cell-Mediated Transfer of Proteoglycan Genes -- Morphological Evaluation of Proteoglycans in Cells and Tissues -- Expression and Characterization of Engineered Proteoglycans -- Intracellular Localization of Engineered Proteoglycans -- Selection of Glycosaminoglycan-Deficient Mutants -- Xyloside Priming of Glycosaminoglycan Biosynthesis and Inhibition of Proteoglycan Assembly -- Inhibition of Heparan Sulfate Synthesis by Chlorate -- Detection of Proteoglycan Core Proteins with Glycosaminoglycan Lyases and Antibodies -- Proteoglycan Core Proteins and Catabolic Fragments Present in Tissues and Fluids -- Degradation of Heparan Sulfate by Nitrous Acid -- Degradation of Heparan Sulfate with Heparin Lyases -- Degradation of Chondroitin Sulfate and Dermatan Sulfate with Chondroitin Lyases -- In Vitro Assay for Hyaluronan Synthase -- Hyaluronidase Activity and Hyaluronidase Inhibitors -- Detecting Hyaluronidase and Hyaluronidase Inhibitors -- Interacations -- Affinity Coelectrophoresis of Proteoglycan-Protein Complexes -- Binding Constant Measurements for Inhibitors of Growth Factor Binding to Heparan Sulfate Proteoglycans -- Interaction of Proteoglycans with Receptor Tyrosine Kinases -- Measuring Single-Cell Cytosolic Ca2+ Concentration in Response to Proteoglycans -- Serum Amyloid A Peptide Interactions with Glycosaminoglycans -- Interactions of Lipoproteins with Proteoglycans -- Hyaluronan and Hyaluronan-Binding Proteins Probes for Specific Detection -- Novel Confocal-FRAP Analysis of Carbohydrate-Protein Interactions Within the Extracellular Matrix -- Regulatory Roles of Syndecans in Cell Adhesion and Invasion -- Optical Biosensor Techniques to Analyze Protein-Polysaccharide Interactions -- Phage Display Technology to Obtain Antiheparan Sulfate Antibodies -- Tissue-Specific Binding by FGF and FGF Receptors to Endogenous Heparan Sulfates.
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| 650 |
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|a Biochemistry.
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| 650 |
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|a Biochemistry, general.
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| 700 |
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|a Iozzo, Renato V.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592592090
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