Proteoglycan Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Edición: | 1st ed. 2001. |
| Colección: | Methods in Molecular Biology,
171 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592592090 |
Tabla de Contenidos:
- Isolation and Purification
- Isolation of Proteoglycans from Cell Cultures and Tissues
- Isolation of Proteoglycans from Tendon
- Purification of Proteoglycans from Mineralized Tissues
- Purification of Perlecan from Endothelial Cells
- Isolation and Characterization of Nervous Tissue Proteoglycans
- Analysis of Proteoglycans and Glycosaminoglycans from Drosophila
- Cartilage and Smooth Muscle Cell Proteoglycans Detected by Affinity Blotting Using Biotinylated Hyaluronan
- Preparation of Proteoglycans for N-Terminal and Internal Amino Acid Sequence Analysis
- High-Specific-Activity 35S-Labeled Heparan Sulfate Prepared from Cultured Cells
- Selective Detection of Sulfotransferase Isoforms by the Ligand Affinity-Conversion Approach
- Particulate and Soluble Glycosaminoglycan-Synthesizing Enzymes
- Disaccharide Composition of Hyaluronan and Chondroitin/Dermatan Sulfate
- Integral Glycan Sequencing of Heparan Sulfate and Heparin Saccharides
- Analytical and Preparative Strong Anion-Exchange HPLC of Heparan Sulfate and Heparin Saccharides
- Proteoglycans Analyzed by Composite Gel Electrophoresis and Immunoblotting
- Quantitation of Proteoglycans in Biological Fluids Using Alcian Blue
- Cellulose Acetate Electrophoresis of Glycosaminoglycans
- Disaccharide Composition in Glycosaminoglycans/Proteoglycans Analyzed by Capillary Zone Electrophoresis
- Intact and Oligomeric Glycosaminoglycans Analyzed with Capillary Electrophoresis
- Expression, Detection, and Degradation
- Recombinant Expression of Proteoglycans in Mammalian Cells
- Purification of Recombinant Human Decorin and Its Subdomains
- Prokaryotic Expression of Proteoglycans
- Proteoglycan Gene Targeting in Somatic Cells
- Constitutive and Inducible Antisense Expression
- Cell-Mediated Transfer of Proteoglycan Genes
- Morphological Evaluation of Proteoglycans in Cells and Tissues
- Expression and Characterization of Engineered Proteoglycans
- Intracellular Localization of Engineered Proteoglycans
- Selection of Glycosaminoglycan-Deficient Mutants
- Xyloside Priming of Glycosaminoglycan Biosynthesis and Inhibition of Proteoglycan Assembly
- Inhibition of Heparan Sulfate Synthesis by Chlorate
- Detection of Proteoglycan Core Proteins with Glycosaminoglycan Lyases and Antibodies
- Proteoglycan Core Proteins and Catabolic Fragments Present in Tissues and Fluids
- Degradation of Heparan Sulfate by Nitrous Acid
- Degradation of Heparan Sulfate with Heparin Lyases
- Degradation of Chondroitin Sulfate and Dermatan Sulfate with Chondroitin Lyases
- In Vitro Assay for Hyaluronan Synthase
- Hyaluronidase Activity and Hyaluronidase Inhibitors
- Detecting Hyaluronidase and Hyaluronidase Inhibitors
- Interacations
- Affinity Coelectrophoresis of Proteoglycan-Protein Complexes
- Binding Constant Measurements for Inhibitors of Growth Factor Binding to Heparan Sulfate Proteoglycans
- Interaction of Proteoglycans with Receptor Tyrosine Kinases
- Measuring Single-Cell Cytosolic Ca2+ Concentration in Response to Proteoglycans
- Serum Amyloid A Peptide Interactions with Glycosaminoglycans
- Interactions of Lipoproteins with Proteoglycans
- Hyaluronan and Hyaluronan-Binding Proteins Probes for Specific Detection
- Novel Confocal-FRAP Analysis of Carbohydrate-Protein Interactions Within the Extracellular Matrix
- Regulatory Roles of Syndecans in Cell Adhesion and Invasion
- Optical Biosensor Techniques to Analyze Protein-Polysaccharide Interactions
- Phage Display Technology to Obtain Antiheparan Sulfate Antibodies
- Tissue-Specific Binding by FGF and FGF Receptors to Endogenous Heparan Sulfates.