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02201nam a22002895i 4500 |
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978-1-59259-210-4 |
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20191027152738.0 |
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cr nn 008mamaa |
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100301s2001 xxu| s |||| 0|eng d |
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|a 9781592592104
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| 024 |
7 |
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|a 10.1385/1592592104
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Two-Hybrid Systems
|b Methods and Protocols /
|c edited by Paul N. MacDonald.
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| 250 |
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|a 1st ed. 2001.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
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| 300 |
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|a XII, 336 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 177
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| 505 |
0 |
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|a General Methods -- The Two-Hybrid System -- Growth and Maintenance of Yeast -- Media Formulations for Various Two-Hybrid Systems -- Yeast Two-Hybrid Vectors and Strains -- High-Efficiency Transformation of Plasmid DNA into Yeast -- Qualitative and Quantitative Assessment of Interactions -- Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies -- False Positives -- Two-Hybrid System and False Positives -- Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns -- Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones -- Applications -- Smad Interactors in Bone Morphogenetic Protein Signaling -- Protein Interactions Important in Eukaryotic Translation Initiation -- Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins -- Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-apoptosis Protein E1B-19K -- Alternative Strategies -- Mammalian Two-Hybrid Assays -- One-Hybrid Systems f hor Detecting Protein-DNA Interactions -- The Split-Hybrid System -- Three-Hybrid Screens -- Three-Hybrid Screens for RNA-Binding Proteins -- Membrane Recruitment Systems for Analysis of Protein-Protein Interactions.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
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|a MacDonald, Paul N.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592592104
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