Two-Hybrid Systems Methods and Protocols /
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Edition: | 1st ed. 2001. |
| Series: | Methods in Molecular Biology,
177 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592592104 |
Table of Contents:
- General Methods
- The Two-Hybrid System
- Growth and Maintenance of Yeast
- Media Formulations for Various Two-Hybrid Systems
- Yeast Two-Hybrid Vectors and Strains
- High-Efficiency Transformation of Plasmid DNA into Yeast
- Qualitative and Quantitative Assessment of Interactions
- Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies
- False Positives
- Two-Hybrid System and False Positives
- Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns
- Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones
- Applications
- Smad Interactors in Bone Morphogenetic Protein Signaling
- Protein Interactions Important in Eukaryotic Translation Initiation
- Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins
- Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-apoptosis Protein E1B-19K
- Alternative Strategies
- Mammalian Two-Hybrid Assays
- One-Hybrid Systems f hor Detecting Protein-DNA Interactions
- The Split-Hybrid System
- Three-Hybrid Screens
- Three-Hybrid Screens for RNA-Binding Proteins
- Membrane Recruitment Systems for Analysis of Protein-Protein Interactions.