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03580nam a22003015i 4500 |
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978-1-59259-248-7 |
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20190618131852.0 |
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cr nn 008mamaa |
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100301s1999 xxu| s |||| 0|eng d |
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|a 9781592592487
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| 024 |
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|a 10.1385/1592592481
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Hemostasis and Thrombosis Protocols
|c edited by David J. Perry, K. John Pasi.
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| 250 |
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|a 1st ed. 1999.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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| 300 |
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|a XIII, 368 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Medicine,
|v 31
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|a Hemostasis -- Basic Techniques -- Isolation of DNA and RNA -- Amplification of DNA and RNA by PCR -- Direct Sequencing of PCR Products -- Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads -- Automated DNA Sequencing -- Detection of DNA by Silver Staining -- Promoter Studies in Hemostasis -- Methods of Mutational Analysis -- Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoresis -- Screening for Mutations in DNA by Single-Stranded Conformation Polymorphism (SSCP) Analysis -- Screening for DNA Heteroduplexes in the Factor VII Gene Using Ethylene Glycol Gel Electrophoresis of Solvent-Treated 32P-Labeled PCR Products -- Detection of Mutations Causing Hemophilia A Using an In Vitro Coupled Transcription and Translation System -- Screening for Mutations in the Human Antithrombin Gene by Hydrolink D-5000™ and MDE™ Gel Electrophoresis -- Methods for Analyzing Inherited/Acquired Disorders of Hemostasis -- Detection of Mutations in Hemophilia A Patients by Chemical Cleavage of Mismatch Method -- Inversion Mutation Analysis in Hemophilia A by Restriction Enzyme Analysis and Southern Blotting -- Hemophilia B Mutational Analysis -- Screening for Candidate Mutations Causing von Willebrand’s Disease (vWD) -- Use of Intron 40 VNTR I in vWD Gene Tracking -- Multimeric Analysis of von Willebrand Factor -- Identification of Mutations in the Human Factor VII Gene -- Molecular Analysis in Factor XI Deficiency -- Mutational Analysis in Antithrombin Deficiency -- Ectopic Transcript Analysis in Human Antithrombin Deficiency -- Mutational Analysis of the Human Protein C Gene -- Analysis of the Protein S Gene in Protein S Deficiency -- Screening for the G to A Transition at Position 20210 in the 3?-Untranslated Region (UTR) of the Prothrombin Gene -- Screening for the Factor V Leiden Mutation -- Multiplex PCR for Detection of the Prothrombin 3?-UTR (G20210A) Polymorphism and the Factor V Leiden Mutation -- Isoelectric Focusing and Immunodetection of Plasma Antithrombin -- Characterization of Heparin Binding Variants of Antithrombin by Crossed Immunoelectrophoresis in the Presence of Heparin -- The Determination of Amino Acid Sequence Abnormalities in Proteins by HPLC Peptide Analysis -- Platelet and Megakaryocyte Analysis -- Molecular Biological Identification and Characterization of Inherited Platelet Receptor Disorders -- In Vitro Expansion of Megakaryocytes from Peripheral Blood Hematopoietic Progenitors -- Molecular Biology Studies with Primary Megakaryocytes.
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| 650 |
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|a Pathology.
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| 650 |
1 |
4 |
|a Pathology.
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| 700 |
1 |
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|a Perry, David J.
|e editor.
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| 700 |
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|a Pasi, K. John.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592592481
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