Hemostasis and Thrombosis Protocols
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
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| Edición: | 1st ed. 1999. |
| Colección: | Methods in Molecular Medicine,
31 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592592481 |
Tabla de Contenidos:
- Hemostasis
- Basic Techniques
- Isolation of DNA and RNA
- Amplification of DNA and RNA by PCR
- Direct Sequencing of PCR Products
- Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads
- Automated DNA Sequencing
- Detection of DNA by Silver Staining
- Promoter Studies in Hemostasis
- Methods of Mutational Analysis
- Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoresis
- Screening for Mutations in DNA by Single-Stranded Conformation Polymorphism (SSCP) Analysis
- Screening for DNA Heteroduplexes in the Factor VII Gene Using Ethylene Glycol Gel Electrophoresis of Solvent-Treated 32P-Labeled PCR Products
- Detection of Mutations Causing Hemophilia A Using an In Vitro Coupled Transcription and Translation System
- Screening for Mutations in the Human Antithrombin Gene by Hydrolink D-5000™ and MDE™ Gel Electrophoresis
- Methods for Analyzing Inherited/Acquired Disorders of Hemostasis
- Detection of Mutations in Hemophilia A Patients by Chemical Cleavage of Mismatch Method
- Inversion Mutation Analysis in Hemophilia A by Restriction Enzyme Analysis and Southern Blotting
- Hemophilia B Mutational Analysis
- Screening for Candidate Mutations Causing von Willebrand’s Disease (vWD)
- Use of Intron 40 VNTR I in vWD Gene Tracking
- Multimeric Analysis of von Willebrand Factor
- Identification of Mutations in the Human Factor VII Gene
- Molecular Analysis in Factor XI Deficiency
- Mutational Analysis in Antithrombin Deficiency
- Ectopic Transcript Analysis in Human Antithrombin Deficiency
- Mutational Analysis of the Human Protein C Gene
- Analysis of the Protein S Gene in Protein S Deficiency
- Screening for the G to A Transition at Position 20210 in the 3?-Untranslated Region (UTR) of the Prothrombin Gene
- Screening for the Factor V Leiden Mutation
- Multiplex PCR for Detection of the Prothrombin 3?-UTR (G20210A) Polymorphism and the Factor V Leiden Mutation
- Isoelectric Focusing and Immunodetection of Plasma Antithrombin
- Characterization of Heparin Binding Variants of Antithrombin by Crossed Immunoelectrophoresis in the Presence of Heparin
- The Determination of Amino Acid Sequence Abnormalities in Proteins by HPLC Peptide Analysis
- Platelet and Megakaryocyte Analysis
- Molecular Biological Identification and Characterization of Inherited Platelet Receptor Disorders
- In Vitro Expansion of Megakaryocytes from Peripheral Blood Hematopoietic Progenitors
- Molecular Biology Studies with Primary Megakaryocytes.