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02995nam a22002895i 4500 |
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978-1-59259-284-5 |
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20191027041351.0 |
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cr nn 008mamaa |
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100301s2002 xxu| s |||| 0|eng d |
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|a 9781592592845
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| 024 |
7 |
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|a 10.1385/1592592848
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Mitochondrial DNA
|b Methods and Protocols /
|c edited by William C. Copeland.
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| 250 |
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|a 1st ed. 2002.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2002.
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| 300 |
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|a XIV, 414 p. 128 illus., 8 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 197
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| 505 |
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|a Methods for the Analysis of MTDNA -- Animal Models for Mitochondrial Disease -- Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases -- Screening for Aging-Dependent Point Mutations in mtDNA -- Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis -- Identification of mtDNA Mutations in Human Cancer -- In Situ PCR of the Common Human mtDNA Deletion -- Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™ -- Yeast Nuclear Genes for mtDNA Maintenance -- Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay -- Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR -- Migration of mtDNA into the Nucleus -- 2D Gel Electrophoresis of mtDNA -- Methods for the Analysis of proteins involved in MTDNA Transactions -- Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions -- Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography -- Characterization of Specialized mtDNA Glycosylases -- Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit -- Assay of mtDNA Polymerase ? from Human Tissues -- Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form -- Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form -- Expression, Purification, and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein -- Recombinant Yeast mtDNA Helicases Purification and Functional Assays -- Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart -- 23 Endonuclease G Isolation and Assays -- 24 Targeting DNA Repair Proteins to Mitochondria -- 25 In Organello Footprinting of mtDNA -- Crosslinking of Proteins to mtDNA -- Breeding and Genotyping of Tfam Conditional Knockout Mice.
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| 650 |
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|a Cell biology.
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| 650 |
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4 |
|a Cell Biology.
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| 700 |
1 |
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|a Copeland, William C.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592592848
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