Mitochondrial DNA Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Copeland, William C. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2002.
Edición:1st ed. 2002.
Colección:Methods in Molecular Biology, 197
Materias:
Acceso en línea:https://doi.org/10.1385/1592592848
Tabla de Contenidos:
  • Methods for the Analysis of MTDNA
  • Animal Models for Mitochondrial Disease
  • Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
  • Screening for Aging-Dependent Point Mutations in mtDNA
  • Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis
  • Identification of mtDNA Mutations in Human Cancer
  • In Situ PCR of the Common Human mtDNA Deletion
  • Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™
  • Yeast Nuclear Genes for mtDNA Maintenance
  • Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay
  • Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR
  • Migration of mtDNA into the Nucleus
  • 2D Gel Electrophoresis of mtDNA
  • Methods for the Analysis of proteins involved in MTDNA Transactions
  • Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions
  • Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography
  • Characterization of Specialized mtDNA Glycosylases
  • Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit
  • Assay of mtDNA Polymerase ? from Human Tissues
  • Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form
  • Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form
  • Expression, Purification, and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein
  • Recombinant Yeast mtDNA Helicases Purification and Functional Assays
  • Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart
  • 23 Endonuclease G Isolation and Assays
  • 24 Targeting DNA Repair Proteins to Mitochondria
  • 25 In Organello Footprinting of mtDNA
  • Crosslinking of Proteins to mtDNA
  • Breeding and Genotyping of Tfam Conditional Knockout Mice.