Mitochondrial DNA Methods and Protocols /
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2002.
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| Edition: | 1st ed. 2002. |
| Series: | Methods in Molecular Biology,
197 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592592848 |
Table of Contents:
- Methods for the Analysis of MTDNA
- Animal Models for Mitochondrial Disease
- Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
- Screening for Aging-Dependent Point Mutations in mtDNA
- Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis
- Identification of mtDNA Mutations in Human Cancer
- In Situ PCR of the Common Human mtDNA Deletion
- Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™
- Yeast Nuclear Genes for mtDNA Maintenance
- Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay
- Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR
- Migration of mtDNA into the Nucleus
- 2D Gel Electrophoresis of mtDNA
- Methods for the Analysis of proteins involved in MTDNA Transactions
- Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions
- Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography
- Characterization of Specialized mtDNA Glycosylases
- Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit
- Assay of mtDNA Polymerase ? from Human Tissues
- Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form
- Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form
- Expression, Purification, and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein
- Recombinant Yeast mtDNA Helicases Purification and Functional Assays
- Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart
- 23 Endonuclease G Isolation and Assays
- 24 Targeting DNA Repair Proteins to Mitochondria
- 25 In Organello Footprinting of mtDNA
- Crosslinking of Proteins to mtDNA
- Breeding and Genotyping of Tfam Conditional Knockout Mice.