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03228nam a22002895i 4500 |
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978-1-59259-342-2 |
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20190618111536.0 |
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cr nn 008mamaa |
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100301s2003 xxu| s |||| 0|eng d |
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|a 9781592593422
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| 024 |
7 |
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|a 10.1385/1592593429
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Protein Sequencing Protocols
|c edited by Bryan John Smith.
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| 250 |
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|a 2nd ed. 2003.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2003.
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| 300 |
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|a XII, 493 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 211
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|a Strategies for Handling Polypeptides on a Micro-Scale -- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing -- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization -- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization -- Enzymatic Cleavage of Proteins -- Chemical Cleavage of Polypeptides -- Enzymatic Preparation and Isolation of Glycopeptides -- Analytical and Micropreparative Capillary Electrophoresis of Peptides -- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues -- Hydrolysis of Samples for Amino Acid Analysis -- Amino Acid Analysis -- Post Column Amino Acid Analysis -- Amino Acid Analysis Using Pre-Column Derivatization with 6-AminoquinolylN-Hydroxysuccinimidyl Carbamate -- Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection -- D-Amino Acid Analysis -- Validation of Amino Acid Analysis Methods -- Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins -- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes -- Direct Identification of Proteins in Ultracomplex Mixtures -- Identification of PTH-Amino Acids by HPLC -- Protein Sequencer Maintenance and Troubleshooting -- N-Terminal Protein Sequencing for Special Applications -- Identification of Phosphorylation Sites by Edman Degradation -- Validation of Protein Sequencing in a Regulated Laboratory -- Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method -- Chemical Modifications of Proteins as an Aid to Sequence Analysis -- Deblocking of N-Terminally Modified Proteins -- Deblocking of Proteins Containing N-Terminal PyroglutamicAcid -- Identification of Sites of Glycosylation -- Analysis of Sites of Protein Phosphorylation -- Quantitation and Location of Disulfide Bonds in Proteins -- Getting the Most from Your Protein Sequence -- Letter Codes, Structures, Masses, and Derivatives of Amino Acids -- Protein Consensus Sequence Motifs.
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| 650 |
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|a Biochemistry.
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| 650 |
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4 |
|a Biochemistry, general.
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| 700 |
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|a Smith, Bryan John.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592593429
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