Protein Sequencing Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2003.
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| Edición: | 2nd ed. 2003. |
| Colección: | Methods in Molecular Biology,
211 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592593429 |
Tabla de Contenidos:
- Strategies for Handling Polypeptides on a Micro-Scale
- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing
- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization
- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization
- Enzymatic Cleavage of Proteins
- Chemical Cleavage of Polypeptides
- Enzymatic Preparation and Isolation of Glycopeptides
- Analytical and Micropreparative Capillary Electrophoresis of Peptides
- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues
- Hydrolysis of Samples for Amino Acid Analysis
- Amino Acid Analysis
- Post Column Amino Acid Analysis
- Amino Acid Analysis Using Pre-Column Derivatization with 6-AminoquinolylN-Hydroxysuccinimidyl Carbamate
- Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection
- D-Amino Acid Analysis
- Validation of Amino Acid Analysis Methods
- Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins
- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry
- Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes
- Direct Identification of Proteins in Ultracomplex Mixtures
- Identification of PTH-Amino Acids by HPLC
- Protein Sequencer Maintenance and Troubleshooting
- N-Terminal Protein Sequencing for Special Applications
- Identification of Phosphorylation Sites by Edman Degradation
- Validation of Protein Sequencing in a Regulated Laboratory
- Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method
- Chemical Modifications of Proteins as an Aid to Sequence Analysis
- Deblocking of N-Terminally Modified Proteins
- Deblocking of Proteins Containing N-Terminal PyroglutamicAcid
- Identification of Sites of Glycosylation
- Analysis of Sites of Protein Phosphorylation
- Quantitation and Location of Disulfide Bonds in Proteins
- Getting the Most from Your Protein Sequence
- Letter Codes, Structures, Masses, and Derivatives of Amino Acids
- Protein Consensus Sequence Motifs.