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04986nam a22003015i 4500 |
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978-1-59259-384-2 |
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20191026232405.0 |
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cr nn 008mamaa |
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100301s2003 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592593842
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| 024 |
7 |
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|a 10.1385/1592593844
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a PCR Protocols
|c edited by John M. S. Bartlett, David Stirling.
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| 250 |
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|a 2nd ed. 2003.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2003.
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| 300 |
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|a 556 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 226
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| 505 |
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|a to PCR -- A Short History of the Polymerase Chain Reaction -- PCR Patent Issues -- Equipping and Establishing a PCR Laboratory -- Quality Control in PCR -- Preparation of Nucleic Acid Templates -- Extraction of Nucleic Acid Templates -- Extraction of DNA from Whole Blood -- DNA Extraction from Tissue -- Extraction of DNA from Microdissected Archival Tissues -- RNA Extraction from Blood -- RNA Extraction from Frozen Tissue -- RNA Extraction from Tissue Sections -- Dual DNA/RNA Extraction -- DNA Extraction from Fungi, Yeast, and Bacteria -- Isolation of RNA Viruses from Biological Materials -- Extraction of Ancient DNA -- DNA Extraction from Plasma and Serum -- Technical Notes for the Detection of Nucleic Acids -- Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels -- Basic PCR Methods -- PCR Primer Design -- Optimization of Polymerase Chain Reactions -- Subcycling PCR for Long-Distance Amplifications of Regions with High and Low Guanine-Cystine Content -- Rapid Amplification of cDNA Ends -- Randomly Amplified Polymorphic DNA Fingerprinting -- Microsphere-Based Single Nucleotide Polymorphism Genotyping -- Ligase Chain Reaction -- Nested RT-PCR in a Single Closed Tube -- Direct PCR from Serum -- Long PCR Amplification of Large Fragments of Viral Genomes -- Long PCR Methodology -- Ultrasensitive and Quantitative PCR -- Qualitative and Quantitative PCR -- Ultrasensitive PCR Detection of Tumor Cells in Myeloma -- Ultrasensitive Quantitative PCR to Detect RNA Viruses -- Quantitative PCR for cAMP RI Alpha mRNA -- Quantitation of Multiple RNA Species -- Transcriptome Analysis -- Differential Display -- AU-Differential Display, Reproducibility of a Differential mRNA Display Targeted to AU Motifs -- PCR Fluorescence Differential Display -- Microarray Analysis Using RNA Arbitrarily Primed PCR -- Oligonucleotide Arrays for Genotyping -- Serial Analysis of Gene Expression -- Mutations and Polymorphisms -- Mutation and Polymorphism Detection -- Combining Multiplex and Touchdown PCR for Microsatellite Analysis -- Detection of Microsatellite Instability and Loss of Heterozygosity Using DNA Extracted from Formalin-Fixed Paraffin-Embedded Tumor Material by Fluorescence-Based Multiplex Microsatellite PCR -- Reduction of Shadow Band Synthesis During PCR Amplification of Repetitive Sequences from Modern and Ancient DNA -- Degenerate Oligonucleotide-Primed PCR -- Mutation Detection Using RT-PCR-RFLP -- Multiplex Amplification Refractory Mutation System for the Detection of Prothrombotic Polymorphisms -- PCR-SSCP Analysis of Polymorphism -- PCR-Based Sequencing -- Sequencing -- Preparation and Direct Automated Cycle Sequencing of PCR Products -- Nonradioactive PCR Sequencing Using Digoxigenin -- Direct Sequencing by Thermal Asymmetric PCR -- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing -- Direct Sequencing with Highly Degenerate and Inosine-Containing Primers -- Determination of Unknown Genomic Sequences Without Cloning -- Cloning PCR Products for Sequencing in M13 Vectors -- DNA Rescue by the Vectorette Method -- Technical Notes for Sequencing Difficult Templates -- In Situ PCR and Prins -- PCR-Based Detection of Nucleic Acids in Chromosomes, Cells, and Tissues -- Cycling Primed In Situ Amplification -- Direct and Indirect In Situ PCR -- Reverse Transcriptase In Situ PCR -- Primed In Situ Nucleic Acid Labeling Combined with Immunocytochemistry to Simultaneously Localize DNA and Proteins in Cells and Chromosomes -- Cloning and Mutagenesis -- Cloning and Mutagenesis -- Using T4 DNA Polymerase to Generate Clonable PCR Products -- A T-Linker Strategy for Modification and Directional Cloning of PCR Products -- Cloning Gene Family Members Using PCR with Degenerate Oligonucleotide Primers -- cDNA Libraries from a Low Amount of Cells -- Creation of Chimeric Junctions, Deletions, and Insertions by PCR -- Recombination and Site-Directed Mutagenesis Using Recombination PCR -- Megaprimer PCR.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Bartlett, John M. S.
|e editor.
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| 700 |
1 |
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|a Stirling, David.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/1592593844
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