PCR Protocols
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2003.
|
| Edición: | 2nd ed. 2003. |
| Colección: | Methods in Molecular Biology,
226 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592593844 |
Tabla de Contenidos:
- to PCR
- A Short History of the Polymerase Chain Reaction
- PCR Patent Issues
- Equipping and Establishing a PCR Laboratory
- Quality Control in PCR
- Preparation of Nucleic Acid Templates
- Extraction of Nucleic Acid Templates
- Extraction of DNA from Whole Blood
- DNA Extraction from Tissue
- Extraction of DNA from Microdissected Archival Tissues
- RNA Extraction from Blood
- RNA Extraction from Frozen Tissue
- RNA Extraction from Tissue Sections
- Dual DNA/RNA Extraction
- DNA Extraction from Fungi, Yeast, and Bacteria
- Isolation of RNA Viruses from Biological Materials
- Extraction of Ancient DNA
- DNA Extraction from Plasma and Serum
- Technical Notes for the Detection of Nucleic Acids
- Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels
- Basic PCR Methods
- PCR Primer Design
- Optimization of Polymerase Chain Reactions
- Subcycling PCR for Long-Distance Amplifications of Regions with High and Low Guanine-Cystine Content
- Rapid Amplification of cDNA Ends
- Randomly Amplified Polymorphic DNA Fingerprinting
- Microsphere-Based Single Nucleotide Polymorphism Genotyping
- Ligase Chain Reaction
- Nested RT-PCR in a Single Closed Tube
- Direct PCR from Serum
- Long PCR Amplification of Large Fragments of Viral Genomes
- Long PCR Methodology
- Ultrasensitive and Quantitative PCR
- Qualitative and Quantitative PCR
- Ultrasensitive PCR Detection of Tumor Cells in Myeloma
- Ultrasensitive Quantitative PCR to Detect RNA Viruses
- Quantitative PCR for cAMP RI Alpha mRNA
- Quantitation of Multiple RNA Species
- Transcriptome Analysis
- Differential Display
- AU-Differential Display, Reproducibility of a Differential mRNA Display Targeted to AU Motifs
- PCR Fluorescence Differential Display
- Microarray Analysis Using RNA Arbitrarily Primed PCR
- Oligonucleotide Arrays for Genotyping
- Serial Analysis of Gene Expression
- Mutations and Polymorphisms
- Mutation and Polymorphism Detection
- Combining Multiplex and Touchdown PCR for Microsatellite Analysis
- Detection of Microsatellite Instability and Loss of Heterozygosity Using DNA Extracted from Formalin-Fixed Paraffin-Embedded Tumor Material by Fluorescence-Based Multiplex Microsatellite PCR
- Reduction of Shadow Band Synthesis During PCR Amplification of Repetitive Sequences from Modern and Ancient DNA
- Degenerate Oligonucleotide-Primed PCR
- Mutation Detection Using RT-PCR-RFLP
- Multiplex Amplification Refractory Mutation System for the Detection of Prothrombotic Polymorphisms
- PCR-SSCP Analysis of Polymorphism
- PCR-Based Sequencing
- Sequencing
- Preparation and Direct Automated Cycle Sequencing of PCR Products
- Nonradioactive PCR Sequencing Using Digoxigenin
- Direct Sequencing by Thermal Asymmetric PCR
- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
- Direct Sequencing with Highly Degenerate and Inosine-Containing Primers
- Determination of Unknown Genomic Sequences Without Cloning
- Cloning PCR Products for Sequencing in M13 Vectors
- DNA Rescue by the Vectorette Method
- Technical Notes for Sequencing Difficult Templates
- In Situ PCR and Prins
- PCR-Based Detection of Nucleic Acids in Chromosomes, Cells, and Tissues
- Cycling Primed In Situ Amplification
- Direct and Indirect In Situ PCR
- Reverse Transcriptase In Situ PCR
- Primed In Situ Nucleic Acid Labeling Combined with Immunocytochemistry to Simultaneously Localize DNA and Proteins in Cells and Chromosomes
- Cloning and Mutagenesis
- Cloning and Mutagenesis
- Using T4 DNA Polymerase to Generate Clonable PCR Products
- A T-Linker Strategy for Modification and Directional Cloning of PCR Products
- Cloning Gene Family Members Using PCR with Degenerate Oligonucleotide Primers
- cDNA Libraries from a Low Amount of Cells
- Creation of Chimeric Junctions, Deletions, and Insertions by PCR
- Recombination and Site-Directed Mutagenesis Using Recombination PCR
- Megaprimer PCR.