Protein Misfolding and Disease
| Corporate Author: | |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2003.
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| Edition: | 1st ed. 2003. |
| Series: | Methods in Molecular Biology,
232 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592593941 |
Table of Contents:
- General Concepts and Models
- Protein Misfolding, Aggregation, and Degradation in Disease
- Basic Introduction to In Vivo Protein Folding and Its Defects
- Cystic Fibrosis
- ?1-Antitrypsin Deficiency
- Parkinson's Disease
- Aberrant Protein Folding as the Molecular Basis of Cancer
- Yeast as a Model System to Investigate Protein Conformational Diseases
- General Methods
- Expression of Recombinant Proteins
- Expression in E. coli Systems
- Protein Expression in Yeasts
- Site-Directed Mutagenesis
- Pulse-Chase Labeling Techniques for the Analysis of Protein Maturation and Degradation
- Techniques in Conformational Disease Research
- Detection of Aggregates and Protein Inclusions by Staining of Tissues
- Study of Mutant Proteins With Folding Defects in Cultured Patient Cells
- E. coli Expression System for Identifying Folding Mutations of Human Adenosine Deaminase
- Characterization of Overexpressed Mutant Proteins in Mammalian Cells
- Investigation of Unfolded-Protein Response in Cells Expressing Familial Alzheimer's Disease-Linked Presenilin Variants
- Huntingtin Fragments Form Aggresome-Like Inclusion Bodies in Mammalian Cells
- Application of Chemical Chaperones to the Rescue of Folding Defects
- Analysis of Defective Subunit Interactions Using the Two-Hybrid System
- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in the Cytosol
- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Microsomes
- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Mitochondria
- Investigation of Folding and Degradation of Mutant Proteins Synthesized in Semipermeabilized Cells.