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03996nam a22002895i 4500 |
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978-1-59259-489-4 |
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20190618041101.0 |
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cr nn 008mamaa |
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100301s1984 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592594894
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| 024 |
7 |
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|a 10.1385/0896030644
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Nucleic Acids
|c edited by John M. Walker.
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| 250 |
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|a 1st ed. 1984.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1984.
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| 300 |
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|a XIV, 375 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 2
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| 505 |
0 |
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|a The Burton Assay for DNA -- DABA Fluorescence Assay for Submicrogram Amounts of DNA -- Preparation of “RNase-Free” DNase by Alkylation -- The Isolation of Satellite DNA by Density Gradient Centrifugation -- The Isolation of High Molecular Weight Eukaryotic DNA -- Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids -- Agarose Gel Electrophoresis of DNA -- Autoradiography of Gels Containing 32P -- Detection of Specific DNA Sequences—The Southern Transfer -- The Extraction and Isolation of DNA from Gels -- One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography -- Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions -- The Extraction of Total RNA by the Detergent and Phenol Method -- RNA Extraction by the Proteinase K Method -- RNA Extraction by the Guanidine Thiocyanate Procedure -- The Purification of Poly(A)-Containing RNA by Affinity Chromatography -- Messenger RNA Fractionation on Neutral Sucrose Gradients -- The Estimation of mRNA Content by Poly(U) Hybridization -- DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts -- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System -- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System -- Immunoprecipitation of in Vitro Translation Products with Protein A Bound to Sepharose -- In Vitro Continuation of RNA Synthesis Initiated in Vivo -- Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA -- Plasmid DNA Isolation by the Cleared Lysate Method -- Plasmid DNA Isolation (Sheared Lysate Method) -- Small-Scale Plasmid DNA Preparation -- Preparation of Chromosomal DNA from E. coli -- Preparation and Assay of Phage Lambda -- Preparation of Phage Lambda DNA -- The Use of Restriction Endonucleases -- Ligation of DNA with T4 DNA Ligase -- The Use of Alkaline Phosphatase to Prevent Vector Regeneration -- Bacterial Transformation -- Bacterial Transformation (Kushner Method) -- In Vitro Packaging of DNA -- Yeast Transformation -- Radiolabeling of DNA by Nick Translation -- Radiolabeling of DNA Using Polynucleotide Kinase -- Radiolabeling of DNA with 3? Terminal Transferase -- Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase -- Identification of Recombinant Plasmids by In Situ Colony Hybridization -- Identification of Recombinant Phages by Plaque Hybridization -- Plasmid Screening Using Single Colony Lysates -- Immunological Detection of Gene Expression in Recombinant Clones -- The Isolation of Minicells -- The Isolation of Maxicells -- The Identification of Gene Products in Minicells and Maxicells -- Molecular Cloning in Bacteriophage Lambda and in Cosmids -- DNA Transformation of Mammalian Cells -- Chemical Cleavage (Maxam and Gilbert) Method for DNA Sequence Determination -- Gel Electrophoretic Analysis of DNA Sequencing Products -- DNA Sequence Determination Using Dideoxy Analogs.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
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|a Walker, John M.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896030644
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