Nucleic Acids
| Autor Corporativo: | |
|---|---|
| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1984.
|
| Edición: | 1st ed. 1984. |
| Colección: | Methods in Molecular Biology,
2 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896030644 |
Tabla de Contenidos:
- The Burton Assay for DNA
- DABA Fluorescence Assay for Submicrogram Amounts of DNA
- Preparation of “RNase-Free” DNase by Alkylation
- The Isolation of Satellite DNA by Density Gradient Centrifugation
- The Isolation of High Molecular Weight Eukaryotic DNA
- Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids
- Agarose Gel Electrophoresis of DNA
- Autoradiography of Gels Containing 32P
- Detection of Specific DNA Sequences—The Southern Transfer
- The Extraction and Isolation of DNA from Gels
- One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography
- Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions
- The Extraction of Total RNA by the Detergent and Phenol Method
- RNA Extraction by the Proteinase K Method
- RNA Extraction by the Guanidine Thiocyanate Procedure
- The Purification of Poly(A)-Containing RNA by Affinity Chromatography
- Messenger RNA Fractionation on Neutral Sucrose Gradients
- The Estimation of mRNA Content by Poly(U) Hybridization
- DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts
- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System
- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System
- Immunoprecipitation of in Vitro Translation Products with Protein A Bound to Sepharose
- In Vitro Continuation of RNA Synthesis Initiated in Vivo
- Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA
- Plasmid DNA Isolation by the Cleared Lysate Method
- Plasmid DNA Isolation (Sheared Lysate Method)
- Small-Scale Plasmid DNA Preparation
- Preparation of Chromosomal DNA from E. coli
- Preparation and Assay of Phage Lambda
- Preparation of Phage Lambda DNA
- The Use of Restriction Endonucleases
- Ligation of DNA with T4 DNA Ligase
- The Use of Alkaline Phosphatase to Prevent Vector Regeneration
- Bacterial Transformation
- Bacterial Transformation (Kushner Method)
- In Vitro Packaging of DNA
- Yeast Transformation
- Radiolabeling of DNA by Nick Translation
- Radiolabeling of DNA Using Polynucleotide Kinase
- Radiolabeling of DNA with 3? Terminal Transferase
- Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
- Identification of Recombinant Plasmids by In Situ Colony Hybridization
- Identification of Recombinant Phages by Plaque Hybridization
- Plasmid Screening Using Single Colony Lysates
- Immunological Detection of Gene Expression in Recombinant Clones
- The Isolation of Minicells
- The Isolation of Maxicells
- The Identification of Gene Products in Minicells and Maxicells
- Molecular Cloning in Bacteriophage Lambda and in Cosmids
- DNA Transformation of Mammalian Cells
- Chemical Cleavage (Maxam and Gilbert) Method for DNA Sequence Determination
- Gel Electrophoretic Analysis of DNA Sequencing Products
- DNA Sequence Determination Using Dideoxy Analogs.