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03145nam a22002895i 4500 |
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978-1-59259-494-8 |
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20190617201452.0 |
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cr nn 008mamaa |
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100301s1991 xxu| s |||| 0|eng d |
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|a 9781592594948
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| 024 |
7 |
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|a 10.1385/0896031780
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Murray, Edward J.
|e author.
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| 245 |
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|a Gene Transfer and Expression Protocols
|c by Edward J. Murray.
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| 250 |
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|a 1st ed. 1991.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1991.
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| 300 |
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|a XIII, 439 p. 33 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 7
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| 505 |
0 |
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|a Preparation of Recombinant Plasmid DNA for DNA-Mediated Gene Transfer -- Calcium Phosphate Mediated Gene Transfer into Established Cell Lines -- Transfection of the ChloramphenicolAcetyltransferase Gene into Eukaryotic Cells Using Diethyl-Aminoethyl (DEAE)-Dextran -- Polybrene/DMSOAssisted Gene Transfer -- Electroporation Technique of DNA Transfection -- Irradiation and Fusion Gene Transfer -- Direct Use of ? Phage Particles for DNA Transfection -- Cationic Liposome-Mediated Transfection with Lipofectin™ Reagent -- Preparation of High-Molecular-Weight DNA for Use in DNA Transfection -- Chromosome-Mediated Gene Thnsfer -- Manipulation of Adenovirus Vectors -- Manipulation of Vaccinia Virus Vectors -- Manipulation of Baculovirus Vectors -- Manipulation of SV40 Vectors -- Choice and Manipulation of Retroviral Vectors -- Use of Tissue-Plasminogen Activator as a Reporter Gene -- Use of Escherichiu coli (E. coli) lacZ (?-Galactosidase) as a Reporter Gene -- Application of the Firefly Luciferase Reporter Gene -- Use of Vectors to Confer Resistance to Antibiotics G418 and Hygromycin in Stably Transfected Cell Lines -- Selection of Cells Defective in Pyrimidine (TK?) and Purine (APRT? and HPRT? Salvage -- Quantitative and Qualitative Analysis of Exogenous Gene Expression by the S1 Nuclease ProtectionAssay -- The RNase Protection Assay -- Primer Extension Analysis of mRNA Isolated from Transfected Cell Lines -- Northern Blot Analysis of Gene Expression -- Analysis of Transcriptional Initiation in Isolated Nuclei -- Immunoperoxidase Staining of Gene Products in Cultured Cells Using Monoclonal Antibodies -- The Use of Flow Cytometry to Detect Transfected Gene Products -- Determination of Foreign Gene Copy Number in Stably Transfected Cell Lines by Southern Transfer Analysis -- Evaluation of Extrachromosomal Gene Copy Number of Transiently Transfected Cell Lines -- Use of Dpn I Restriction Enzyme to Assess Newly Replicated Gene Copies in Amplifiable Vector Systems -- Use of Polymerase Chain Reaction (PCR) to Detect Homologous Recombination in Transfected Cell Lines -- Induction of Erythroid-Specific Expression in Murine Erythroleukemia (MEL) Cell Lines.
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| 650 |
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|a Human genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896031780
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