Gene Transfer and Expression Protocols
| Main Author: | |
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| Corporate Author: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1991.
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| Edition: | 1st ed. 1991. |
| Series: | Methods in Molecular Biology,
7 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/0896031780 |
Table of Contents:
- Preparation of Recombinant Plasmid DNA for DNA-Mediated Gene Transfer
- Calcium Phosphate Mediated Gene Transfer into Established Cell Lines
- Transfection of the ChloramphenicolAcetyltransferase Gene into Eukaryotic Cells Using Diethyl-Aminoethyl (DEAE)-Dextran
- Polybrene/DMSOAssisted Gene Transfer
- Electroporation Technique of DNA Transfection
- Irradiation and Fusion Gene Transfer
- Direct Use of ? Phage Particles for DNA Transfection
- Cationic Liposome-Mediated Transfection with Lipofectin™ Reagent
- Preparation of High-Molecular-Weight DNA for Use in DNA Transfection
- Chromosome-Mediated Gene Thnsfer
- Manipulation of Adenovirus Vectors
- Manipulation of Vaccinia Virus Vectors
- Manipulation of Baculovirus Vectors
- Manipulation of SV40 Vectors
- Choice and Manipulation of Retroviral Vectors
- Use of Tissue-Plasminogen Activator as a Reporter Gene
- Use of Escherichiu coli (E. coli) lacZ (?-Galactosidase) as a Reporter Gene
- Application of the Firefly Luciferase Reporter Gene
- Use of Vectors to Confer Resistance to Antibiotics G418 and Hygromycin in Stably Transfected Cell Lines
- Selection of Cells Defective in Pyrimidine (TK?) and Purine (APRT? and HPRT? Salvage
- Quantitative and Qualitative Analysis of Exogenous Gene Expression by the S1 Nuclease ProtectionAssay
- The RNase Protection Assay
- Primer Extension Analysis of mRNA Isolated from Transfected Cell Lines
- Northern Blot Analysis of Gene Expression
- Analysis of Transcriptional Initiation in Isolated Nuclei
- Immunoperoxidase Staining of Gene Products in Cultured Cells Using Monoclonal Antibodies
- The Use of Flow Cytometry to Detect Transfected Gene Products
- Determination of Foreign Gene Copy Number in Stably Transfected Cell Lines by Southern Transfer Analysis
- Evaluation of Extrachromosomal Gene Copy Number of Transiently Transfected Cell Lines
- Use of Dpn I Restriction Enzyme to Assess Newly Replicated Gene Copies in Amplifiable Vector Systems
- Use of Polymerase Chain Reaction (PCR) to Detect Homologous Recombination in Transfected Cell Lines
- Induction of Erythroid-Specific Expression in Murine Erythroleukemia (MEL) Cell Lines.