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03598nam a22002895i 4500 |
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978-1-59259-502-0 |
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20191026142244.0 |
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cr nn 008mamaa |
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100301s1993 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595020
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| 024 |
7 |
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|a 10.1385/0896032442
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a PCR Protocols
|b Current Methods and Applications /
|c edited by Bruce A. White.
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| 250 |
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|a 1st ed. 1993.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1993.
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| 300 |
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|a XIV, 392 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 15
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| 505 |
0 |
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|a Polymerase Chain Reaction -- Selection of Primers for Polymerase Chain Reaction -- Direct Radioactive Labeling of Polymerase Chain Reaction Products -- Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays -- Nonradioactive Labeling of Polymerase Chain Reaction Products -- Quantitation and Purification of Polymerase Chain Reaction Products by High-Performance Liquid Chromatography -- Use of Polymerase Chain Reaction for Screening Transgenic Mice -- Polymerase Chain Reaction Analysis of DNA from Paraffin-Embedded Tissue -- The Use of Polymerase Chain Reaction for Chromosome Assignment -- Mapping MHC Class II Genes and Disease-Susceptibility -- The Use of the Polymerase Chain Reaction and the Detection of Amplified Products -- Determination of Loss of Heterozygosity Using Polymerase Chain Reaction -- Direct Sequencing of Polymerase Chain Reaction Products -- Manual and Automated Direct Sequencing of Product Generated by the Polymerase Chain Reaction -- Genomic Footprinting by Ligation Mediated Polymerase Chain Reaction -- RNA Template-Specific Polymerase Chain Reaction (RS-PCR) -- Quantitative Measurement of Relative Gene Expression in Human Tumors -- Identification of Alternatively Spliced mRNAs and Localization of 5' Ends by Polymerase Chain Reaction Amplification -- Utilization of Polymerase Chain Reaction for Clonal Analysis of Gene Expression -- Sequencing DNA Amplified Directly from a Bacterial Colony -- Use of Polymerase Chain Reaction to Screen Phage Libraries -- Molecular Cloning of Polymerase Chain Reaction Fragments with Cohesive Ends -- Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products -- Use of Polymerase Chain Reaction for Making Recombinant Constructs -- In Vitro Recombination and Mutagenesis of DNA -- Use of Polymerase Chain Reaction for the Rapid Construction of Synthetic Genes -- Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis -- Site-Directed Mutagenesis by Double Polymerase Chain Reaction -- Generation of a Polymerase Chain Reaction Renewable Source of Subtractive cDNA -- PCR-Based Full-Length cDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-Pair Strategy -- Use of Degenerate Oligonucleotide Primers and the Polymerase Chain Reaction to Clone Gene Family Members -- Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking -- cDNA Cloning by Inverse Polymerase Chain Reaction -- Amplification of Gene Ends from Gene Libraries by Polymerase Chain Reaction with Single-Sided Specificity -- Anchoring a Defined Sequence to the 5? Ends of mRNAs.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a White, Bruce A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
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|u https://doi.org/10.1385/0896032442
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