PCR Protocols Current Methods and Applications /

PCR Protocols Current Methods and Applications /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: White, Bruce A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 1993.
Edición:1st ed. 1993.
Colección:Methods in Molecular Biology, 15
Materias:
Cell biology.
Cell Biology.
Acceso en línea:https://doi.org/10.1385/0896032442
Tabla de Contenidos:
  • Polymerase Chain Reaction
  • Selection of Primers for Polymerase Chain Reaction
  • Direct Radioactive Labeling of Polymerase Chain Reaction Products
  • Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays
  • Nonradioactive Labeling of Polymerase Chain Reaction Products
  • Quantitation and Purification of Polymerase Chain Reaction Products by High-Performance Liquid Chromatography
  • Use of Polymerase Chain Reaction for Screening Transgenic Mice
  • Polymerase Chain Reaction Analysis of DNA from Paraffin-Embedded Tissue
  • The Use of Polymerase Chain Reaction for Chromosome Assignment
  • Mapping MHC Class II Genes and Disease-Susceptibility
  • The Use of the Polymerase Chain Reaction and the Detection of Amplified Products
  • Determination of Loss of Heterozygosity Using Polymerase Chain Reaction
  • Direct Sequencing of Polymerase Chain Reaction Products
  • Manual and Automated Direct Sequencing of Product Generated by the Polymerase Chain Reaction
  • Genomic Footprinting by Ligation Mediated Polymerase Chain Reaction
  • RNA Template-Specific Polymerase Chain Reaction (RS-PCR)
  • Quantitative Measurement of Relative Gene Expression in Human Tumors
  • Identification of Alternatively Spliced mRNAs and Localization of 5' Ends by Polymerase Chain Reaction Amplification
  • Utilization of Polymerase Chain Reaction for Clonal Analysis of Gene Expression
  • Sequencing DNA Amplified Directly from a Bacterial Colony
  • Use of Polymerase Chain Reaction to Screen Phage Libraries
  • Molecular Cloning of Polymerase Chain Reaction Fragments with Cohesive Ends
  • Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products
  • Use of Polymerase Chain Reaction for Making Recombinant Constructs
  • In Vitro Recombination and Mutagenesis of DNA
  • Use of Polymerase Chain Reaction for the Rapid Construction of Synthetic Genes
  • Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis
  • Site-Directed Mutagenesis by Double Polymerase Chain Reaction
  • Generation of a Polymerase Chain Reaction Renewable Source of Subtractive cDNA
  • PCR-Based Full-Length cDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-Pair Strategy
  • Use of Degenerate Oligonucleotide Primers and the Polymerase Chain Reaction to Clone Gene Family Members
  • Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking
  • cDNA Cloning by Inverse Polymerase Chain Reaction
  • Amplification of Gene Ends from Gene Libraries by Polymerase Chain Reaction with Single-Sided Specificity
  • Anchoring a Defined Sequence to the 5? Ends of mRNAs.

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