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03183nam a22002895i 4500 |
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978-1-59259-508-2 |
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20190617194227.0 |
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cr nn 008mamaa |
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100301s1993 xxu| s |||| 0|eng d |
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|a 9781592595082
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| 024 |
7 |
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|a 10.1385/0896032396
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Hyde, John E.
|e author.
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| 245 |
1 |
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|a Protocols in Molecular Parasitology
|c by John E. Hyde.
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| 250 |
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|a 1st ed. 1993.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1993.
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| 300 |
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|a XIV, 470 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 21
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| 505 |
0 |
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|a Culturing and Biological Cloning of Trypanosoma brucei -- Culturing and Biological Cloning of Trypanosoma cruzi -- In Vitro Cultivation and Biological Cloning of Leishmania -- Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages) -- Synchronization and Cloning of Malaria Parasites -- The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies -- The Culture of Schistosoma mansoni and Production of Life Cycle Stages -- Preparation of DNA and RNA from Trypanosoma brucei -- Isolation of RNA and DNA from Trypanosoma cruzi -- Isolation of DNA and RNA from Leishmania -- The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum -- Extraction and Purification of Nucleic Acids from Schistosomes -- Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting -- The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification -- Analysis of the DNA of Parasitic Protozoa by Flow Cytometry -- PCR orLowLevel Detection of Malaria Parasites in Blood -- PCR Methods for Identification of Point Mutations and Gene Rearrangements -- Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques -- Ribosomal RNA Probes for Detection and Identification of Species -- Rapid Sequencing of Parasite rRNA -- Antibody Screening of Expression Libraries -- Antibody Select Procedure for Characterization of Expression Clones -- Isolation of Parasite Genes Using Synthetic Oligonucleotides -- Chromosome Mapping Methods for Parasitic Protozoa -- Transfection ofLeishmania and Trypanosoma brucei by Electroporation -- Two-Dimensional Polyacrylamide Gel Electrophoresis -- Isoenzyme Electrophoresis for Parasite Characterization -- The Surface Labeling of Schistosomes -- Monoclonal Antibody Affinity Chromatography -- Immunoblotting and Enzyme-Linked Immunosorbent Assay -- Immunofluorescence of Parasites -- Immunoelectron Microscopic Localization of Antigens in Malaria Parasites -- Preparation of Blotted Membrane for Protein Microsequencing -- Identification of T-Cell Epitopes.
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| 650 |
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|a Microbiology.
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| 650 |
1 |
4 |
|a Microbiology.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896032396
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