Protocols in Molecular Parasitology
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| Corporate Author: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1993.
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| Edition: | 1st ed. 1993. |
| Series: | Methods in Molecular Biology,
21 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/0896032396 |
Table of Contents:
- Culturing and Biological Cloning of Trypanosoma brucei
- Culturing and Biological Cloning of Trypanosoma cruzi
- In Vitro Cultivation and Biological Cloning of Leishmania
- Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages)
- Synchronization and Cloning of Malaria Parasites
- The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies
- The Culture of Schistosoma mansoni and Production of Life Cycle Stages
- Preparation of DNA and RNA from Trypanosoma brucei
- Isolation of RNA and DNA from Trypanosoma cruzi
- Isolation of DNA and RNA from Leishmania
- The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum
- Extraction and Purification of Nucleic Acids from Schistosomes
- Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting
- The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification
- Analysis of the DNA of Parasitic Protozoa by Flow Cytometry
- PCR orLowLevel Detection of Malaria Parasites in Blood
- PCR Methods for Identification of Point Mutations and Gene Rearrangements
- Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques
- Ribosomal RNA Probes for Detection and Identification of Species
- Rapid Sequencing of Parasite rRNA
- Antibody Screening of Expression Libraries
- Antibody Select Procedure for Characterization of Expression Clones
- Isolation of Parasite Genes Using Synthetic Oligonucleotides
- Chromosome Mapping Methods for Parasitic Protozoa
- Transfection ofLeishmania and Trypanosoma brucei by Electroporation
- Two-Dimensional Polyacrylamide Gel Electrophoresis
- Isoenzyme Electrophoresis for Parasite Characterization
- The Surface Labeling of Schistosomes
- Monoclonal Antibody Affinity Chromatography
- Immunoblotting and Enzyme-Linked Immunosorbent Assay
- Immunofluorescence of Parasites
- Immunoelectron Microscopic Localization of Antigens in Malaria Parasites
- Preparation of Blotted Membrane for Protein Microsequencing
- Identification of T-Cell Epitopes.