|
|
|
|
| LEADER |
03572nam a22002895i 4500 |
| 001 |
978-1-59259-515-0 |
| 005 |
20191027032436.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s1994 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781592595150
|
| 024 |
7 |
|
|a 10.1385/089603254X
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 100 |
1 |
|
|a Isaac, Peter G.
|e author.
|
| 245 |
1 |
0 |
|a Protocols for Nucleic Acid Analysis by Nonradioactive Probes
|c by Peter G. Isaac.
|
| 250 |
|
|
|a 1st ed. 1994.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1994.
|
| 300 |
|
|
|a XII, 268 p.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 28
|
| 505 |
0 |
|
|a About Nonradioactive Nucleic Acid Detection -- Isolation of DNA from Plants -- Isolation of High-Molecular-Weight DNA from Animal Cells -- Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes -- Isolation of Plant RNA -- Isolation of Total and Poly A+ RNA from Animal Cells -- Preparation of RNA Gel Blots -- Preparation of RNA Dot Blots -- Isolation of Plasmids for the Preparation of Probes -- Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides -- Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotide Priming -- Digoxigenin Labeling of RNA Transcripts from Multi- and Single-Locus DNA Minisatellite Probes -- Labeling of Double-Stranded DNA Probes with Biotin -- Preparation of Horseradish Peroxidase-Labeled Probes -- Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP -- Labeling of Oligonucleotides with Fluorescein -- Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence -- Southern Blot Hybridization of Digoxigenin-Labeled RNA Minisatellite Probes and Color Detection -- Hybridization and Detection of Digoxigenin Probes on RNA Blots -- Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence -- Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Enhanced Chemiluminescence -- Hybridization of Fluorescein-Labeled Oligonucleotide Probes and Enhanced Chemiluminescence Detection -- Preparation of Chromosome Spreads by Root-Tip Meristem Dissection for In Situ Hybridization with Biotin-Labeled Probes -- Enzymatic Treatment of Plant Material to Spread Chromosomes for In Situ Hybridization -- Use of Biotin-Labeled Probes on Plant Chromosomes -- Direct Fluorochrome-Labeled DNA Probes for Direct Fluorescent In Situ Hybridization to Chromosomes -- Detection of Digoxigenin-Labeled DNA Probes Hybridized to Plant Chromosomes In Situ -- Preparation of Tissue Sections and Slides for mRNA Hybridization -- Detecting mRNAin Tissue Sections with Digoxigenin-Labeled Probes -- Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes -- PACE (Probe Assay—Chemiluminescence Enhanced) -- Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format -- Analysis of Gene Sequences by Hybridization of PCR-Amplified DNA to Covalently Bound Oligonucleotide Probes -- RAPD Assay -- Nonradioactive Oligonucleotide Probes for Detecting Products of the Ligase Chain Reaction -- Nucleic Acid Sequence-Based Amplification (NASBA™).
|
| 650 |
|
0 |
|a Cell biology.
|
| 650 |
1 |
4 |
|a Cell Biology.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/089603254X
|