Protocols for Nucleic Acid Analysis by Nonradioactive Probes
| Autor principal: | |
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| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1994.
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| Edición: | 1st ed. 1994. |
| Colección: | Methods in Molecular Biology,
28 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/089603254X |
Tabla de Contenidos:
- About Nonradioactive Nucleic Acid Detection
- Isolation of DNA from Plants
- Isolation of High-Molecular-Weight DNA from Animal Cells
- Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes
- Isolation of Plant RNA
- Isolation of Total and Poly A+ RNA from Animal Cells
- Preparation of RNA Gel Blots
- Preparation of RNA Dot Blots
- Isolation of Plasmids for the Preparation of Probes
- Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides
- Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotide Priming
- Digoxigenin Labeling of RNA Transcripts from Multi- and Single-Locus DNA Minisatellite Probes
- Labeling of Double-Stranded DNA Probes with Biotin
- Preparation of Horseradish Peroxidase-Labeled Probes
- Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP
- Labeling of Oligonucleotides with Fluorescein
- Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence
- Southern Blot Hybridization of Digoxigenin-Labeled RNA Minisatellite Probes and Color Detection
- Hybridization and Detection of Digoxigenin Probes on RNA Blots
- Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence
- Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Enhanced Chemiluminescence
- Hybridization of Fluorescein-Labeled Oligonucleotide Probes and Enhanced Chemiluminescence Detection
- Preparation of Chromosome Spreads by Root-Tip Meristem Dissection for In Situ Hybridization with Biotin-Labeled Probes
- Enzymatic Treatment of Plant Material to Spread Chromosomes for In Situ Hybridization
- Use of Biotin-Labeled Probes on Plant Chromosomes
- Direct Fluorochrome-Labeled DNA Probes for Direct Fluorescent In Situ Hybridization to Chromosomes
- Detection of Digoxigenin-Labeled DNA Probes Hybridized to Plant Chromosomes In Situ
- Preparation of Tissue Sections and Slides for mRNA Hybridization
- Detecting mRNAin Tissue Sections with Digoxigenin-Labeled Probes
- Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes
- PACE (Probe Assay—Chemiluminescence Enhanced)
- Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format
- Analysis of Gene Sequences by Hybridization of PCR-Amplified DNA to Covalently Bound Oligonucleotide Probes
- RAPD Assay
- Nonradioactive Oligonucleotide Probes for Detecting Products of the Ligase Chain Reaction
- Nucleic Acid Sequence-Based Amplification (NASBA™).